The dark side of foetal bovine serum in extracellular vesicle studies

O Urzì, R Olofsson Bagge… - Journal of extracellular …, 2022 - Wiley Online Library
Extracellular vesicles (EVs) have been shown to be involved in cell‐cell communication and
to take part in both physiological and pathological processes. Thanks to their exclusive …

[HTML][HTML] Foetal bovine serum influence on in vitro extracellular vesicle analyses

BM Lehrich, Y Liang, MS Fiandaca - Journal of Extracellular …, 2021 - ncbi.nlm.nih.gov
Extracellular vesicles (EVs) are nanosized lipid bilayer vesicles most notably from either
endosomal (ie, exosomes) or plasma membrane origins (ie, microvesicles/ectosomes) and …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

L Dong, RC Zieren, K Horie, CJ Kim… - Journal of …, 2020 - Wiley Online Library
One of the challenges that restricts the evolving extracellular vesicle (EV) research field is
the lack of a consensus method for EV separation. This may also explain the diversity of the …

[HTML][HTML] A shared, stochastic pathway mediates exosome protein budding along plasma and endosome membranes

FK Fordjour, C Guo, Y Ai, GG Daaboul… - Journal of Biological …, 2022 - ASBMB
Exosomes are small extracellular vesicles of∼ 30 to 150 nm that are secreted by all cells,
abundant in all biofluids, and play important roles in health and disease. However, details …

Small extracellular vesicles deliver miR-21 and miR-217 as pro-senescence effectors to endothelial cells

E Mensà, M Guescini, A Giuliani… - Journal of …, 2020 - Taylor & Francis
The role of epigenetics in endothelial cell senescence is a cutting-edge topic in ageing
research. However, little is known of the relative contribution to pro-senescence signal …

MSC‐derived exosomes‐based therapy for peripheral nerve injury: a novel therapeutic strategy

R Dong, Y Liu, Y Yang, H Wang, Y Xu… - BioMed research …, 2019 - Wiley Online Library
Although significant advances have been made in synthetic nerve conduits and surgical
techniques, complete regeneration following peripheral nerve injury (PNI) remains far from …

Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays

S Busatto, Y Yang, D Iannotta… - Journal of …, 2022 - Wiley Online Library
With an exponential increase in extracellular vesicle (EV) studies in the past decade, focus
has been placed on standardization of experimental design to ensure inter‐study …

Advances in analytical technologies for extracellular vesicles

H Yan, Y Li, S Cheng, Y Zeng - Analytical Chemistry, 2021 - ACS Publications
Sensitive early detection, along with effective treatments and solid postoperative monitoring
remain the grand challenges in fighting against human diseases, especially cancer.(1) In …

Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters

F Shekari, FJ Alibhai, H Baharvand… - Journal of …, 2023 - Wiley Online Library
Cell culture‐conditioned medium (CCM) is a valuable source of extracellular vesicles (EVs)
for basic scientific, therapeutic and diagnostic applications. Cell culturing parameters affect …