Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

Sortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA),
the best studied sortase class, plays a key role in anchoring surface proteins with the …

Enzymatic site-specific bioconjugation techniques, in particular sortase-mediated ligation,
are increasingly used to generate conjugated proteins for a wide array of applications …

Directed sortase A evolution yielded the variants R159G and D165Q/D186G/K196V with
increased resistance (2.2-fold) and catalytic efficiency (6.3-fold) in 45%(v/v) …

Numerous enzymes of biotechnological importance have been immobilized on magnetic
nanoparticles (MNP) via random multipoint attachment, resulting in a heterogeneous protein …

Wild‐type sortase A is an important virulence factor displaying a diverse array of proteins on
the surface of bacteria. This protein display relies on the transpeptidase activity of sortase A …

Sortase‐mediated ligation (SML) has become a powerful tool for site‐specific protein
modification. However, sortase A (SrtA) suffers from low catalytic efficiency and mediates an …

Sortase A is a virulence factor responsible for the attachment of surface proteins to
Staphylococcus aureus and other Gram-positive bacteria. Inhibitors of this enzyme are …

Direct benzene hydroxylation under mild conditions using hydrogen peroxide can be
essential in producing phenol. A new iron oxide-carbonaceous nanocatalyst (Fe-oxide …

The immobilization of proteins on nanoparticles has received much attention in recent years.
Among different approaches, enzymatic protein immobilization shows unique advantages …