[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives

S Ahmar, G Hensel, D Gruszka - Biotechnology Advances, 2023 - Elsevier
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing

X Tang, Y Zhang - New Phytologist, 2023 - Wiley Online Library
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …

Efficient and multiplex gene upregulation in plants through CRISPR-Cas-mediated knockin of enhancers

Q Yao, R Shen, Y Shao, Y Tian, P Han, X Zhang… - Molecular plant, 2024 - cell.com
Gene upregulation through genome editing is important for plant research and breeding.
Targeted insertion of short transcriptional enhancers (STEs) into gene promoters may offer a …

Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Z Zhong, G Liu, Z Tang, S Xiang, L Yang… - Nature …, 2023 - nature.com
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …

Genome-edited foods

A Tuncel, C Pan, T Sprink, R Wilhelm… - Nature Reviews …, 2023 - nature.com
Genome editing can transform agriculture and shape the future of food by improving crop
yields and animal productivity, which in turn can help to achieve food security for the …

Rewiring gene circuitry for plant improvement

AT Borowsky, J Bailey-Serres - Nature Genetics, 2024 - nature.com
Aspirations for high crop growth and yield, nutritional quality and bioproduction of materials
are challenged by climate change and limited adoption of new technologies. Here, we …

The pan-tandem repeat map highlights multiallelic variants underlying gene expression and agronomic traits in rice

H He, Y Leng, X Cao, Y Zhu, X Li, Q Yuan… - Nature …, 2024 - nature.com
Tandem repeats (TRs) are genomic regions that tandemly change in repeat number, which
are often multiallelic. Their characteristics and contributions to gene expression and …

Promoter editing for the genetic improvement of crops

L Shi, J Su, MJ Cho, H Song, X Dong… - Journal of …, 2023 - academic.oup.com
Gene expression plays a fundamental role in the regulation of agronomically important traits
in crop plants. The genetic manipulation of plant promoters through genome editing has …

Targeted genome-modification tools and their advanced applications in crop breeding

B Li, C Sun, J Li, C Gao - Nature Reviews Genetics, 2024 - nature.com
Crop improvement by genome editing involves the targeted alteration of genes to improve
plant traits, such as stress tolerance, disease resistance or nutritional content. Techniques …

Engineering a plant A-to-K base editor with improved performance by fusion with a transactivation module

Y Li, S Li, C Li, C Zhang, L Yan, J Li, Y He, Y Guo… - Plant …, 2023 - cell.com
Dear Editor, 1 Base editors (BEs) are promising tools enabling a single nucleotide
substitution in a 2 specific target gene to generate either loss-of-function or gain-of-function …