Calibrating evanescent-wave penetration depths for biological TIRF microscopy
Roughly half of a cell's proteins are located at or near the plasma membrane. In this
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …
restricted space, the cell senses its environment, signals to its neighbors, and exchanges …
Fluorescence imaging with tailored light
Fluorescence microscopy has long been a valuable tool for biological and medical imaging.
Control of optical parameters such as the amplitude, phase, polarization, and propagation …
Control of optical parameters such as the amplitude, phase, polarization, and propagation …
Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane
Total internal reflection fluorescence (TIRF) microscopy offers powerful means to uncover
the functional organization of proteins in the plasma membrane with very high spatial and …
the functional organization of proteins in the plasma membrane with very high spatial and …
E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment
In many non-excitable cells, the depletion of endoplasmic reticulum (ER) Ca2+ stores leads
to the dynamic formation of membrane contact sites (MCSs) between the ER and the plasma …
to the dynamic formation of membrane contact sites (MCSs) between the ER and the plasma …
Structured illumination microscopy using digital micro-mirror device and coherent light source
Structured illumination microscopy (SIM) achieves doubled spatial resolution through
exciting the specimen with high-contrast, high-frequency sinusoidal patterns. Such an …
exciting the specimen with high-contrast, high-frequency sinusoidal patterns. Such an …
Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells
Single-molecule super-resolution imaging is instrumental in investigating cellular
architecture and organization at the nanoscale. Achieving precise 3D nanometric …
architecture and organization at the nanoscale. Achieving precise 3D nanometric …
Spinning-spot shadowless TIRF microscopy
KL Ellefsen, JL Dynes, I Parker - PLoS One, 2015 - journals.plos.org
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-
membrane cellular structures and processes, including imaging of local Ca2+ transients with …
membrane cellular structures and processes, including imaging of local Ca2+ transients with …
Clusters of a few bound cofilins sever actin filaments
JP Bibeau, S Gray, M Enrique - Journal of molecular biology, 2021 - Elsevier
Cofilin is an essential actin filament severing protein that accelerates the assembly
dynamics and turnover of actin networks by increasing the number of filament ends where …
dynamics and turnover of actin networks by increasing the number of filament ends where …
Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells
Single-molecule localization microscopy (SMLM) techniques, such as single-molecule
tracking (SMT), enable in situ measurements in cells from which data-rich metrics can be …
tracking (SMT), enable in situ measurements in cells from which data-rich metrics can be …
Shaping an evanescent focus of light for high spatial resolution optogenetic activations in live cells
M Grosjean, A Grichine, M Pezet, O Destaing… - Optics …, 2024 - opg.optica.org
Confining light illumination in the three dimensions of space is a challenge for various
applications. Among these, optogenetic methods developed for live experiments in cell …
applications. Among these, optogenetic methods developed for live experiments in cell …