Organ‐on‐a‐chip platforms: a convergence of advanced materials, cells, and microscale technologies

S Ahadian, R Civitarese, D Bannerman… - Advanced …, 2018 - Wiley Online Library
Significant advances in biomaterials, stem cell biology, and microscale technologies have
enabled the fabrication of biologically relevant tissues and organs. Such tissues and organs …

Quantitative two-photon imaging of fluorescent biosensors

G Yellen, R Mongeon - Current opinion in chemical biology, 2015 - Elsevier
Highlights•Two-photon imaging of biosensors in intact tissue is challenging to
calibrate.•Ratiometric or fluorescence lifetime imaging can provide the needed …

Sequential ionic and conformational signaling by calcium channels drives neuronal gene expression

B Li, MR Tadross, RW Tsien - Science, 2016 - science.org
Voltage-gated CaV1. 2 channels (L-type calcium channel α1C subunits) are critical
mediators of transcription-dependent neural plasticity. Whether these channels signal via …

Quantifying macromolecular interactions in living cells using FRET two-hybrid assays

ES Butz, M Ben-Johny, M Shen, PS Yang, L Sang… - Nature protocols, 2016 - nature.com
Förster resonance energy transfer (FRET) is a versatile method for analyzing protein–protein
interactions within living cells. This protocol describes a nondestructive live-cell FRET assay …

Tools, methods, and applications for optophysiology in neuroscience

N Smedemark-Margulies, JG Trapani - Frontiers in molecular …, 2013 - frontiersin.org
The advent of optogenetics and genetically encoded photosensors has provided
neuroscience researchers with a wealth of new tools and methods for examining and …

GABA transporter function, oligomerization state, and anchoring: correlates with subcellularly resolved FRET

FJ Moss, PI Imoukhuede, K Scott, J Hu… - Journal of General …, 2009 - rupress.org
The mouse γ-aminobutyric acid (GABA) transporter mGAT1 was expressed in
neuroblastoma 2a cells. 19 mGAT1 designs incorporating fluorescent proteins were …

FRET detection of amyloid β-peptide oligomerization using a fluorescent protein probe presenting a pseudo-amyloid structure

T Takahashi, H Mihara - Chemical Communications, 2012 - pubs.rsc.org
FRET detection of amyloid β- peptide oligomerization using a fluorescent protein probe
presenting a pseudo-amyloid structure - Chemical Communications (RSC Publishing) DOI:10.1039/C1CC14552E …

Development of mKate3/HaloTag7 (JFX650) and CFP/YFP Dual-Fluorescence (or Förster) Resonance Energy Transfer Pairs for Visualizing Dual-Molecular Activity

W Wang, J Yang - ACS sensors, 2024 - ACS Publications
Although several imaging strategies for dual fluorescence (or Förster) resonance energy
transfer (FRET) biosensors have been reported, their implementation is challenging …

FRET spectroscopy—towards effective biomolecular probing

EM Obeng, EC Dullah, MK Danquah, C Budiman… - Analytical …, 2016 - pubs.rsc.org
The advent of super-resolution microscopy has been a major breakthrough in bioscience
research, allowing accurate molecular signaling analysis of cellular and biological materials …

RhoA is down-regulated at cell–cell contacts via p190RhoGAP-B in response to tensional homeostasis

SM Ponik, SM Trier, MA Wozniak… - Molecular biology of …, 2013 - Am Soc Cell Biol
Breast epithelial cells cultured in three-dimensional (3D) collagen gels undergo ductal
morphogenesis when the gel is compliant and they can achieve tensional homeostasis. We …