4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector

A Bucci, G Tortarolo, MO Held, L Bega… - Nature …, 2024 - nature.com
Single-particle tracking techniques enable investigation of the complex functions and
interactions of individual particles in biological environments. Many such techniques exist …

Single‐molecule fluorescence microscopy for imaging chemical reactions: Recent progress and future opportunities for advancing polymer systems

CB Dunn, S Valdez, Z Qiang - Journal of Polymer Science, 2024 - Wiley Online Library
Single‐molecule fluorescence (smFL) imaging techniques have evolved greatly over the
past two decades to encompass the ability to monitor chemical reactions, providing unique …

Targeted Single Particle Tracking with Upconverting Nanoparticles

O Dukhno, S Ghosh, V Greiner, S Bou… - … Applied Materials & …, 2024 - ACS Publications
Single particle tracking (SPT) is a powerful technique for real-time microscopic visualization
of the movement of individual biomolecules within or on the surface of living cells. However …

Information optimization of laser scanning microscopes for real-time feedback-driven single particle tracking

NA Vickers, F Sharifi, SB Andersson - Optics Express, 2023 - opg.optica.org
Real-time feedback-driven single particle tracking (RT-FD-SPT) is a class of microscopy
techniques that uses measurements of finite excitation/detection volume in a feedback …

Active-feedback 3D single-molecule tracking using a fast-responding galvo scanning mirror

X Tan, S Hou, A Niver, C Zhang… - The Journal of …, 2023 - ACS Publications
Real-time three-dimensional single-particle tracking (RT-3D-SPT) allows continuous
detection of individual freely diffusing objects with high spatiotemporal precision by applying …

Theoretical comparison of real-time feedback-driven single-particle tracking techniques

B Van Heerden, TPJ Krüger - The Journal of chemical physics, 2022 - pubs.aip.org
Real-time feedback-driven single-particle tracking is a technique that uses feedback control
to enable single-molecule spectroscopy of freely diffusing particles in native or near-native …

3D Real-Time Two-Photon Microscopy Device for Single-Particle Holographic Tracking (3D-Red Shot)

F Semmer, MC Emperauger, C Lopez… - ACS …, 2023 - ACS Publications
Three-dimensional real-time tracking of single light emitters is an emerging tool for the
assessment of biological processes such as axonal transport, for which high spatiotemporal …

Biophysical Reviews' “Meet the IUPAB councillor series”: a brief profile of Tjaart PJ Krüger

TPJ Krüger - Biophysical Reviews, 2024 - Springer
It is a great privilege for me to have been elected to the IUPAB Council earlier this year and
to represent the African continent on the Council. In this short commentary, I am giving a very …

Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments

AJ Niver, KD Welsher - The Journal of Chemical Physics, 2022 - pubs.aip.org
Despite successes in tracking single molecules in vitro, the extension of active-feedback
single-particle methods to tracking rapidly diffusing and unconfined proteins in live cells has …

4D Single-Particle Tracking with Asynchronous Read-Out SPAD-Array Detector

A Bucci, G Tortarolo, MO Held, L Bega, E Perego… - BioRxiv, 2023 - biorxiv.org
Single-particle tracking (SPT) techniques are essential for investigating the complex
functions and interactions of individual, specifically labelled particles in biological …