[PDF][PDF] Foreign gene expression in yeast: a review

MA Romanos, CA Scorer, JJ Clare - Yeast, 1992 - static.igem.org
YIP vectors Transplacement Integration into reiterated DNA Transposition vectors
Transcriptional promoters and terminators Foreign versus yeast promoters Glycolytic …

Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae

J Hou, KEJ Tyo, Z Liu, D Petranovic… - FEMS Yeast …, 2012 - academic.oup.com
The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels
and chemicals, and it is also provides a platform for the production of many heterologous …

Transforming growth factor α: mutation of aspartic acid 47 and leucine 48 results in different biological activities

E Lazar, S Watanabe, S Dalton… - Molecular and Cellular …, 1988 - Taylor & Francis
To study the relationship between the primary structure of transforming growth factor α (TGF-
α) and some of its functional properties (competition with epidermal growth factor (EGF) for …

Yeast secretory expression of insulin precursors

T Kjeldsen - Applied microbiology and biotechnology, 2000 - Springer
Since the 1980s, recombinant human insulin for the treatment of diabetes mellitus has been
produced using either the yeast Saccharomyces cerevisiae or the prokaryote Escherichia …

A novel leader peptide which allows efficient secretion of a fragment of human interleukin 1 beta in Saccharomyces cerevisiae.

C Baldari, JA Murray, P Ghiara, G Cesareni… - The EMBO …, 1987 - embopress.org
Killer strains of Kluyveromyces lactis secrete a toxin which presumably is processed during
secretion from a larger precursor. Analysis of the sequence of the K. lactis killer toxin gene …

Heterologous protein secretion from yeast

RA Smith, MJ Duncan, DT Moir - Science, 1985 - science.org
Secretion of calf prochymosin from yeast yields fully activable zymogen while production in
the yeast cytoplasm yields insoluble, unactivable enzyme with aberrant disulfide bonding …

Saccharomyces cerevisiae as host for the recombinant production of polyketides and nonribosomal peptides

A Tippelt, M Nett - Microbial Cell Factories, 2021 - Springer
As a robust, fast growing and genetically tractable organism, the budding yeast
Saccharomyces cerevisiae is one of the most widely used hosts in biotechnology. Its …

Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast

P Aza, G Molpeceres, F de Salas… - Cellular and Molecular Life …, 2021 - Springer
Saccharomyces cerevisiae plays an important role in the heterologous expression of an
array of proteins due to its easy manipulation, low requirements and ability for protein post …

Prepro-alpha-factor has a cleavable signal sequence.

MG Waters, EA Evans, G Blobel - Journal of biological chemistry, 1988 - Elsevier
MAT alpha Saccharomyces cerevisiae secrete a small peptide mating pheromone termed
alpha-factor. Its precursor, prepro-alpha-factor, is translocated into the endoplasmic …

[PDF][PDF] Vector systems for the expression, analysis and cloning of DNA sequence in S. cerevisiae

SA Parent, CM Fenimore, KA Bostian - Yeast, 1985 - academia.edu
Our understanding of many cellular and molecular phenomena in the yeast Succharomyces
cerevisiae has increased dramatically over the past seven years due largely to the …