The manufacturing of recombinant protein is traditionally divided in two main steps:
upstream (cell culture and synthesis of the target protein) and downstream (purification and …

Highlights•The current status of continuous cell culture and downstream processes is
presented.•The impact of continuous operation on product quality is shown.•Resulting …

Over twenty-five years ago, perfusion was applied to mammalian cells to produce
therapeutic proteins, mainly sensitive ones. Nowadays, other issues such as process …

Highlights•Nucleotide sugars are precursors to the sugars involved in protein N-
glycosylation.•Different media supplements, such as N-acetylmannosamine and uridine …

A two-step procedure for the design and optimization of mammalian cell perfusion cultures
for therapeutic protein production, for a given expression system, is developed. The …

Process control strategies based on the physiological status of cells have recently been
used to enhance mammalian cell culture productivity and robustness. In this study, we …

Master the design and operation of perfusion cell cultures with this authoritative reference.
Discover the current state-of-the-art in the design and operation of continuous bioreactors …

This chapter aims to put forth a range of examples for continuous control of the most
commonly used unit operations in manufacturing of monoclonal antibodies (mAbs) …

Biologically manufactured monoclonal antibodies (mAb) can strongly vary in their efficacy
and affinity. Therefore, engineering and production of the mAb is highly regulated and …

Clinical and commercial success of therapeutic proteins has been ever increasing in the
past decades. New product classes are emerging while biosimilars, the equivalent of small …