Multiplexed DNA and Protease Detection with Orthogonal Energy Transfer on a Single Quantum Dot Scaffolded Biosensor

DA Hastman, S Hooe, M Chiriboga, SA Díaz… - ACS …, 2023 - ACS Publications
Almost all pathogens, whether viral or bacterial, utilize key proteolytic steps in their
pathogenesis. The ability to detect a pathogen's genomic material along with its proteolytic …

An mRNA-Based Multiple Antigenic Gene Expression System Delivered by Engineered Salmonella for Severe Fever with Thrombocytopenia Syndrome and …

JY Park, C Hewawaduge, C Sivasankar, KKS Lloren… - Pharmaceutics, 2023 - mdpi.com
Currently, there are no commercial vaccines or therapeutics against severe fever with
thrombocytopenia syndrome (SFTS) virus. This study explored an engineered Salmonella …

Novel Stable Protease Inhibitor from Phoenix dactylifera (L.) Flowers with Antimicrobial and Antitumoral Activities

I Jemel, N Krayem, A Ben Bacha, M Alonazi… - ACS …, 2024 - ACS Publications
A novel protease inhibitor isolated from date palm Phoenix dactylifera (L.) flowers (PIDF)
was purified and characterized. A heat and acidic treatment step followed by ethanol …

Genome-Assisted Probiotic Characterization and Application of Lactiplantibacillus plantarum 18 as a Candidate Probiotic for Laying Hen Production

G Zhang, N Yang, Z Liu, X Chen, M Li, T Fu, D Zhang… - Microorganisms, 2023 - mdpi.com
Probiotics gained significant attention for their potential to improve gut health and enhance
productivity in animals, including poultry. This comprehensive study focused on the genetic …

Lon1 Inactivation Downregulates Autophagic Flux and Brassinosteroid Biogenesis, Modulating Mitochondrial Proportion and Seed Development in Arabidopsis

C Song, Y Hou, T Li, Y Liu, XA Wang, W Qu… - International Journal of …, 2024 - mdpi.com
Mitochondrial protein homeostasis is crucially regulated by protein degradation processes
involving both mitochondrial proteases and cytosolic autophagy. However, it remains …

[HTML][HTML] Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis

M Li, H Liu, KY Hsieh, S Zhang, Y Gao, Q Gong… - Fundamental …, 2024 - Elsevier
The Lon proteases are evolutionarily conserved oligomeric hydrolases characterized by a
built-in ATPase module. Here, we report the structures of LonC from Meiothermus …

Sensing endogenous RNA in living human cells using a CRISPR-activated protease

K Blumenstock, A Hoch, LD Hinterlang, CI Fandrey… - bioRxiv, 2024 - biorxiv.org
Most techniques used to detect specific mRNAs in eukaryotic cells require to extract nucleic
acids and thereby kill the cells. A programmable sensor for monitoring endogenous …

Identification of ClpS Mediated N-End Rule Degradation in Mycolicibacterium smegmatis

CJ Presloid - 2023 - search.proquest.com
Tuberculosis (TB) has been and remains one of the most significant human infectious
diseases, and currently causes more global death than any other single infectious agent …