An invitation to measure insect cold tolerance: methods, approaches, and workflow

BJ Sinclair, LEC Alvarado, LV Ferguson - Journal of Thermal Biology, 2015 - Elsevier
Insect performance is limited by the temperature of the environment, and in temperate, polar,
and alpine regions, the majority of insects must face the challenge of exposure to low …

The integrative physiology of insect chill tolerance

J Overgaard, HA MacMillan - Annual review of physiology, 2017 - annualreviews.org
Cold tolerance is important in defining the distribution of insects. Here, we review the
principal physiological mechanisms underlying homeostatic failure during cold exposure in …

How to assess Drosophila cold tolerance: chill coma temperature and lower lethal temperature are the best predictors of cold distribution limits

JL Andersen, T Manenti, JG Sørensen… - Functional …, 2015 - Wiley Online Library
Thermal tolerance may limit and therefore predict ectotherm geographic distributions.
However, which of the many metrics of thermal tolerance best predict distribution is often …

Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress

R Grgac, J Rozsypal, L Des Marteaux… - Proceedings of the …, 2022 - National Acad Sciences
Most multicellular organisms are freeze sensitive, but the ability to survive freezing of the
extracellular fluids evolved in several vertebrate ectotherms, some plants, and many insects …

Insect capa neuropeptides impact desiccation and cold tolerance

S Terhzaz, NM Teets, P Cabrero… - Proceedings of the …, 2015 - National Acad Sciences
The success of insects is linked to their impressive tolerance to environmental stress, but
little is known about how such responses are mediated by the neuroendocrine system. Here …

The capacity to maintain ion and water homeostasis underlies interspecific variation in Drosophila cold tolerance

HA MacMillan, JL Andersen, SA Davies, J Overgaard - Scientific reports, 2015 - nature.com
Many insects, including Drosophila, succumb to the physiological effects of chilling at
temperatures well above those causing freezing. Low temperature causes a loss of …

Osmoregulatory capacity at low temperature is critical for insect cold tolerance

J Overgaard, L Gerber, MK Andersen - Current Opinion in Insect Science, 2021 - Elsevier
At low temperature many insects lose extracellular ion homeostasis and the capacity to
mitigate homeostatic imbalance determines their cold tolerance. Extracellular homeostasis …

Cold-induced depolarization of insect muscle: differing roles of extracellular K+ during acute and chronic chilling

HA MacMillan, A Findsen… - Journal of …, 2014 - journals.biologists.com
Insects enter chill coma, a reversible state of paralysis, at temperatures below their critical
thermal minimum (CTmin), and the time required for an insect to recover after a cold …

Rapid cold hardening: ecological relevance, physiological mechanisms and new perspectives

NM Teets, JD Gantz… - Journal of Experimental …, 2020 - journals.biologists.com
Rapid cold hardening (RCH) is a type of phenotypic plasticity that allows ectotherms to
quickly enhance cold tolerance in response to brief chilling (lasting minutes to hours). In this …

The central nervous system and muscular system play different roles for chill coma onset and recovery in insects

MK Andersen, J Overgaard - … and Physiology Part A: Molecular & …, 2019 - Elsevier
When insects are cooled, they initially lose their ability to perform coordinated movements at
their critical thermal minima (CT min). At a slightly lower temperature, they enter a state of …