Improving the stability of proteins is an important goal in many biomedical and industrial
applications. A logical approach is to emulate stabilizing molecular interactions found in …

Cancer is one of the most common diseases and causes of death worldwide. Since common
treatment approaches do not yield acceptable results in many patients, developing …

Background Disulfide engineering is an important biotechnological tool that has advanced a
wide range of research. The introduction of novel disulfide bonds into proteins has been …

Nanobodies represent the variable binding domain of camelid heavy-chain antibodies and
are employed in a rapidly growing range of applications in biotechnology and biomedicine …

Highlights•Ancestral environments likely differed from the environments hosting modern
proteins.•Resurrected ancestral protein often display unusual properties and …

Thermostabilization represents a critical and often obligatory step toward enhancing the
robustness of enzymes for organic synthesis and other applications. While directed …

Enhancing the thermostability of enzymes without impacting their catalytic function
represents an important yet challenging goal in protein engineering and biocatalysis. We …

Abstract Lipase Lip2 from Yarrowia lipolytica with great potential value in the enzymatic
preparation of medium-long-medium structured lipids (MLM-SLs), but the synthetic efficiency …

Phytase is one of the most important feed additive enzymes for monogastric animal,
because it hydrolyzes the indigestible phytate in the cereal‐based feedstock to release …

Diacylglycerols (DAGs) display huge application prospectives in food industries. Therefore,
new strategies to produce diacylglycerides are needed. Malassezia globose lipase (SMG1) …