CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …

Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …

Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but
therapeutic applications require efficient cargo loading. Here, we developed new methods …

CRISPR-Cas9 genome editing has promising therapeutic potential for genetic diseases and
cancers, but safety could be a concern. Here we use whole genomic analysis by 10x linked …

Cancer is one of the leading causes of death worldwide and it has the trend of increase
incidence. However, in the past decades, as quickly developed new technologies and …

Nanopore long-read sequencing is an emerging approach for studying genomes, including
long repetitive elements like telomeres. Here, we report extensive basecalling induced …

CRISPR/Cas9 has been adapted to disrupt endogenous genes in adoptive T-lymphocyte
therapy to prevent graft-versus-host disease. However, genome editing also generates …

Recent advancements in RNA therapeutics highlight the critical need for precision gene
delivery systems that target specific organs and cells. Lipid nanoparticles (LNPs) have …

As an emerging treatment strategy for malignant tumors, chimeric antigen receptor T (CAR-
T) cell therapy has been widely used in clinical practice, and its efficacy has been markedly …