Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

The molecular organization of eukaryotic nuclear volumes remains largely unexplored. Here
we combined recent developments in cryo–electron tomography (cryo-ET) to produce three …

Macromolecular crowding has a profound impact on reaction rates and the physical
properties of the cell interior, but the mechanisms that regulate crowding are poorly …

Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …

Cryo-focused ion beam milling of frozen-hydrated cells has recently provided
unprecedented insights into the inner space of cells. In combination with cryo-electron …

Cellular electron cryotomography offers researchers the ability to observe macromolecules
frozen in action in situ, but a primary challenge with this technique is identifying molecular …

Macromolecular complexes are intrinsically flexible and often challenging to purify for
structure determination by single-particle cryo-electron microscopy (cryo-EM). Such …

Electron cryotomography is currently the only method capable of visualizing cells in three
dimensions at nanometer resolutions. While modern instruments produce massive amounts …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …