Horseradish peroxidase has been the subject of scientific research for centuries. It has been
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …

The heterologous expression of biosynthetic pathways for pharmaceutical or fine chemical
production requires suitable expression hosts and vectors. In eukaryotes, the pathway flux is …

Polyploidization can provide a wealth of genetic variation for adaptive evolution and
speciation, but understanding the mechanisms of subgenome evolution as well as its …

Methylotrophic yeasts such as Komagataella phaffii (syn. Pichia pastoris, Pp), Hansenula
polymorpha (Hp), Candida boidinii (Cb) and Pichia methanolica (Pm) are widely used …

Silymarin is a collection of compounds extracted from the medicinal herb milk thistle, among
which silybin is the major flavonolignan. However, the biosynthesis pathway of silybin …

Fighting cancer still relies on chemo-and radiation therapy, which is a trade-off between
effective clearance of malignant cells and severe side effects on healthy tissue. Targeted …

Silybin and isosilybin are the major active components of silymarin, which have long been
used to treat liver disease. Market available silymarin is currently extracted from the seed of …

We have developed efficient procedure for isolation of horseradish peroxidase (HRP) using
aminobenzohydrazide‐based affinity chromatography. Sepharose 4B‐bounded …

Horseradish peroxidase (HRP) is a high-demand enzyme for applications in diagnostics,
bioremediation, biocatalysis and medicine. Current HRP preparations are isolated from …

Background Tagging proteins is a standard method facilitating protein detection, purification
or targeting. When tagging a certain protein of interest, it is challenging to predict which tag …