Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides
(glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate …

Polysaccharides are ubiquitous biomolecules found in nature that contain various biological
and pharmacological activities that are employed in functional foods and therapeutic agents …

N-glycosylation of proteins is now routinely characterized and monitored because of its
significance to the detection of disease states and the manufacturing of biopharmaceuticals …

1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …

We present a detailed protocol for the structural analysis of protein-linked glycans. In this
approach, appropriate for glycomics studies, N-linked glycans are released using peptide N …

Glycosylation is among the most important post-translational modifications for proteins and
is of intrinsic complex character compared with DNAs and naked proteins. Indeed, over 50 …

Carbohydrates display a large diversity of structures and their analysis presents many
obstacles as the result of properties such as isomeric diversity, existence of branched …

Mass spectrometry (MS) of glycoproteins is an emerging field in proteomics, poised to meet
the technical demand for elucidation of the structural complexity and functions of the …

Glycosylation defines the adhesive properties of animal cell surfaces and the surrounding
extracellular environments. Because cells respond to stimuli by altering glycan expression …

A miniaturized approach was developed for quantitative permethylation of oligosaccharides,
which involves packing of sodium hydroxide powder in microspin columns or fused‐silica …