Mobile genetic elements (MGEs) are crucial for horizontal gene transfer (HGT) in bacteria
and facilitate their rapid evolution and adaptation. MGEs include plasmids, integrative and …

Cell physiology requires innumerable metalloenzymes supported by the selective import of
metal ions. Within the crowded cytosol, most enzymes acquire their cognate cofactors from a …

Abstract pET expression plasmids are widely used for producing recombinant proteins in
Escherichia coli. Selection and maintenance of cells harboring a pET plasmid are possible …

The increasing threat of antibiotic resistance and shrinking treatment options for infections
have pushed mankind into a difficult position. The looming threat of the return of the pre …

Abstract β-Lactamases are bacterial enzymes that inactivate β-lactam antibiotics and, as
such, are the most prevalent cause of antibiotic resistance in Gram-negative bacteria. The …

Klebsiella pneumoniae is a ubiquitous opportunistic pathogen of the family
Enterobacteriaceae. K. pneumoniae is a member of the ESKAPEE pathogens (Enterococcus …

The growing worldwide epidemic of antibiotic resistance demands a better understanding of
the primary molecular pathways, with stress response systems emerging as critical players …

Nocardia are Gram-positive bacteria from the Actinobacteria phylum. Some Nocardia
species can infect humans and are usually considered to be opportunist pathogens, as they …

This review addresses the involvement of DNA supercoiling in the development of virulence
and antibiotic profiles for uropathogenic Escherichia coli and the emergence of new …

Mycobacteria are intrinsically resistant to beta-lactams as they possess several putative
penicillin-interactive enzymes (PIEs), some of those are with dual-activity, namely DD …