Yeast surface display system: strategies for improvement and biotechnological applications

KV Teymennet-Ramírez, F Martínez-Morales… - … in Bioengineering and …, 2022 - frontiersin.org
Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression
of proteins immobilized on the yeast's cell surface. YSD combines the advantages …

Biodegradation of highly crystallized poly (ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin

Z Chen, R Duan, Y Xiao, Y Wei, H Zhang, X Sun… - Nature …, 2022 - nature.com
The process of recycling poly (ethylene terephthalate)(PET) remains a major challenge due
to the enzymatic degradation of high-crystallinity PET (hcPET). Recently, a bacterial PET …

Integrated Catalytic Upgrading of Biomass-Derived Alcohols for Advanced Biofuel Production

S Shanmugam, A Hari, A Pugazhendhi, T Kikas - Energies, 2023 - mdpi.com
Sustainable biofuel production is necessary to meet the daunting challenge of “fueling”
growing economies with a significantly reduced carbon footprint. Although its higher oxygen …

Engineered repeat proteins as scaffolds to assemble multi-enzyme systems for efficient cell-free biosynthesis

A Ledesma-Fernandez, S Velasco-Lozano… - Nature …, 2023 - nature.com
Multi-enzymatic cascades with enzymes arranged in close-proximity through a protein
scaffold can trigger a substrate channeling effect, allowing for efficient cofactor reuse with …

DeepEnzyme: a robust deep learning model for improved enzyme turnover number prediction by utilizing features of protein 3D-structures

T Wang, G Xiang, S He, L Su, Y Wang… - Briefings in …, 2024 - academic.oup.com
Turnover numbers (k cat), which indicate an enzyme's catalytic efficiency, have a wide range
of applications in fields including protein engineering and synthetic biology. Experimentally …

One-pot chemoenzymatic reactions in water enabled by micellar encapsulation

CJ Hastings, NP Adams, J Bushi, SJ Kolb - Green Chemistry, 2020 - pubs.rsc.org
The use of micellar conditions to enable one-pot reactions involving both transition metal
and enzymatic catalysts is reported. Representative enzymatic transformations under …

Co-immobilization of an enzyme system on a metal-organic framework to produce a more effective biocatalyst

R Ahmad, J Shanahan, S Rizaldo, DS Kissel, KL Stone - Catalysts, 2020 - mdpi.com
In many respects, enzymes offer advantages over traditional chemical processes due to their
decreased energy requirements for function and inherent greener processing. However …

Direct yeast surface codisplay of sequential enzymes with complementary anchor motifs: enabling enhanced glycosylation of natural products

F Guo, M Liu, H Liu, C Li, X Feng - ACS Synthetic Biology, 2023 - ACS Publications
Yeast surface display is an appealing technique for constructing multienzyme cascades.
This technique is commonly achieved using a scaffold for the ordered arrangement of …

Solid-phase assembly of multienzyme systems into artificial cellulosomes

N Zeballos, E Diamanti, AI Benítez-Mateos… - Bioconjugate …, 2021 - ACS Publications
We herein describe a bioinspired solid-phase assembly of a multienzyme system scaffolded
on an artificial cellulosome. An alcohol dehydrogenase and an ω-transaminase were fused …

Cascade chiral amine synthesis catalyzed by site-specifically co-immobilized alcohol and amine dehydrogenases

S Liu, Z Wang, K Chen, L Yu, Q Shi, X Dong… - Catalysis Science & …, 2022 - pubs.rsc.org
Chiral amines are valuable intermediates widely applied in the pharmaceutical industry. As
an alternative to classical synthetic methods for the production of chiral amines, biocatalytic …