The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and
widely used methodology for biological investigation because it can detect and quantify very …

Practical Handbook of Microbiology, 4th edition provides basic, clear and concise
knowledge and practical information about working with microorganisms. Useful to anyone …

Completely revised, this third edition of a bestseller incorporates findings to present readers
with a complete overview of foodborne listeriosis, including information on listeriosis in …

Listeria monocytogenes is an important food-borne pathogen and is widely tested for in
food, environmental and clinical samples. Identification traditionally involved culture …

In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has
become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of …

Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable
Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect …

We monitored the dynamic changes in the bacterial population in milk associated with
refrigeration. Direct analyses of DNA by using temporal temperature gel electrophoresis …

We developed and assessed real-time PCR (RTi-PCR) assays for the detection and
quantification of the food-borne pathogen Listeria monocytogenes and the closely related …

The objective of this study was to develop a method combining nanoparticle-based
immunomagnetic separation (IMS) with real-time PCR for a rapid and quantitative detection …