Considerations for the recovery of recombinant proteins from plants

TJ Menkhaus, Y Bai, C Zhang, ZL Nikolov… - Biotechnology …, 2004 - Wiley Online Library
The past 5 years have seen the commercialization of two recombinant protein products from
transgenic plants, and many recombinant therapeutic proteins produced in plants are …

Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins

MTW Hearn, D Acosta - Journal of Molecular Recognition, 2001 - Wiley Online Library
In this article, recent progress related to the use of different types of polypeptide fusion
handles or 'tags' for the purification of recombinant proteins are critically discussed. In …

One‐step purification, covalent immobilization, and additional stabilization of poly‐His‐tagged proteins using novel heterofunctional chelate‐epoxy supports

C Mateo, G Fernández‐Lorente… - Biotechnology and …, 2001 - Wiley Online Library
Epoxy supports covalently immobilize proteins following a two-step mechanism; that is, the
protein is physically adsorbed and then the covalent reaction takes place. This mechanism …

Chromatographic separation of proteins on metal immobilized iminodiacetic acid-bound molded monolithic rods of macroporous poly (glycidyl methacrylate–co …

Q Luo, H Zou, X Xiao, Z Guo, L Kong, X Mao - Journal of Chromatography A, 2001 - Elsevier
Continuous rod of macroporous poly (glycidyl methacrylate–co-ethylene dimethacrylate)
was prepared by a free radical polymerization within the confines of a stainless-steel …

Facile fabrication of hydrophilic nanofibrous membranes with an immobilized metal–chelate affinity complex for selective protein separation

J Zhu, G Sun - ACS applied materials & interfaces, 2014 - ACS Publications
In this study, we report a facile approach to fabricate functionalized poly (vinyl alcohol-co-
ethylene)(PVA-co-PE) nanofibrous membranes as immobilized metal affinity membranes for …

Immobilization of horseradish peroxidase on hierarchically porous magnetic metal-organic frameworks for visual detection and efficient degradation of 2, 4 …

X Gao, H Pan, K Yang, J Dang, C Qiao, Y Liu… - Biochemical Engineering …, 2023 - Elsevier
Immobilization of enzymes onto solid carrier is an important way to improve the stability and
reusability of enzymes. However, the enzymatic activity and catalytic efficiency are severely …

Surface recognition of a protein using designed transition metal complexes

MA Fazal, BC Roy, S Sun, S Mallik… - Journal of the American …, 2001 - ACS Publications
Each protein has a unique pattern of histidine residues on the surface. This paper describes
the design, synthesis, and binding studies of transition metal complexes to target the surface …

Affinity chromatography of polyhistidine tagged enzymes: New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired …

C Mateo, G Fernandez-Lorente, BCC Pessela… - … of Chromatography A, 2001 - Elsevier
New immobilized metal ion affinity chromatography (IMAC) matrices containing a high
concentration of metal–chelate moieties and completely coated with inert flexible and …

Hyaluronic acid hydrogel loaded with genetically-engineered brain-derived neurotrophic factor as a neural cell carrier

T Nakaji-Hirabayashi, K Kato, H Iwata - Biomaterials, 2009 - Elsevier
Cell transplantation is a potential therapy for central nervous disorders such as Parkinson's
disease. However, the therapeutic effect is limited by the low viability of transplanted cells …

Enhanced protein delivery from photopolymerized hydrogels using a pseudospecific metal chelating ligand

CC Lin, AT Metters - Pharmaceutical research, 2006 - Springer
Purpose This study was conducted to investigate the cause of incomplete protein release
from photopolymerized poly (ethylene glycol)(PEG) hydrogels and verify the protein …