Microbial indicators of fecal pollution: recent progress and challenges in assessing water quality
DA Holcomb, JR Stewart - Current environmental health reports, 2020 - Springer
Abstract Purpose of Review Fecal contamination of water is a major public health concern.
This review summarizes recent developments and advancements in water quality indicators …
This review summarizes recent developments and advancements in water quality indicators …
[HTML][HTML] Comparing the significance of the utilization of next generation and third generation sequencing technologies in microbial metagenomics
Since the exploration of sequencing began in 2005, third and next-generation sequencing
(TGS and NGS) technologies have fundamentally changed metagenomics research. These …
(TGS and NGS) technologies have fundamentally changed metagenomics research. These …
The newest Oxford Nanopore R10. 4.1 full-length 16S rRNA sequencing enables the accurate resolution of species-level microbial community profiling
T Zhang, H Li, S Ma, J Cao, H Liao… - Applied and …, 2023 - Am Soc Microbiol
The long-read amplicon provides a species-level solution for the community. With the
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …
improvement of nanopore flowcells, the accuracy of Oxford Nanopore Technologies (ONT) …
Using sewage for surveillance of antimicrobial resistance
FM Aarestrup, MEJ Woolhouse - Science, 2020 - science.org
Antimicrobial resistance (AMR), a cross-cutting and increasing threat to global health (1–3),
is a complex problem with multiple and interconnected drivers. Reliable surveillance data …
is a complex problem with multiple and interconnected drivers. Reliable surveillance data …
Nanopore is preferable over Illumina for 16S amplicon sequencing of the gut microbiota when species-level taxonomic classification, accurate estimation of richness …
M Szoboszlay, L Schramm, D Pinzauti, J Scerri… - Microorganisms, 2023 - mdpi.com
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …
Freshwater monitoring by nanopore sequencing
While traditional microbiological freshwater tests focus on the detection of specific bacterial
indicator species, including pathogens, direct tracing of all aquatic DNA through …
indicator species, including pathogens, direct tracing of all aquatic DNA through …
[HTML][HTML] Metagenomic water quality monitoring with a portable laboratory
We describe the technical feasibility of metagenomic water quality analysis using only
portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges …
portable equipment, for example mini-vacuum pumps and filtration units, mini-centrifuges …
Expanding the temporal and spatial scales of environmental DNA research with autonomous sampling
Environmental DNA (eDNA) is an emerging and powerful method for use in marine
research, conservation, and management, yet time‐and resource‐intensive protocols limit …
research, conservation, and management, yet time‐and resource‐intensive protocols limit …
Next generation sequencing for pathogen detection in periprosthetic joint infections
PF Indelli, S Ghirardelli, B Violante… - EFORT Open …, 2021 - eor.bioscientifica.com
Periprosthetic joint infections (PJI) represent one of the most catastrophic complications
following total joint arthroplasty (TJA). The lack of standardized diagnostic tests and …
following total joint arthroplasty (TJA). The lack of standardized diagnostic tests and …
Speeding up the detection of invasive bivalve species using environmental DNA: A Nanopore and Illumina sequencing comparison
Traditional detection of aquatic invasive species via morphological identification is often time‐
consuming and can require a high level of taxonomic expertise, leading to delayed …
consuming and can require a high level of taxonomic expertise, leading to delayed …