The spatiotemporal control of gene expression is dependent on the activity of cis-acting
regulatory sequences, called enhancers, which regulate target genes over variable genomic …

How enhancers control target gene expression over long genomic distances remains an
important unsolved problem. Here we investigated enhancer–promoter communication by …

Virtually all cell types have the same DNA, yet each type exhibits its own cell-specific pattern
of gene expression. During the brief period of mitosis, the chromosomes exhibit changes in …

The complex and dynamic three-dimensional organization of chromatin within the nucleus
makes understanding the control of gene expression challenging, but also opens up …

Enhancers are DNA sequences that enable complex temporal and tissue-specific regulation
of genes in higher eukaryotes. Although it is not entirely clear how enhancer-promoter …

During self-renewal, cell-type-defining features are drastically perturbed in mitosis and must
be faithfully reestablished upon G1 entry, a process that remains largely elusive. Here, we …

Mammalian embryogenesis commences with two pivotal and binary cell fate decisions that
give rise to three essential lineages: the trophectoderm, the epiblast and the primitive …

Cell fate transitions observed in embryonic development involve changes in three-
dimensional genomic organization that provide proper lineage specification. Whether similar …

Mechanisms of tissue-specific gene expression regulation via 3D genome organization are
poorly understood. Here we uncover the regulatory chromatin network of developing T cells …

Current methods for analyzing chromatin architecture are not readily scalable to
heterogeneous tissues. Here we introduce Droplet Hi-C, which uses a commercial …