Selective enzymes separation from the fermentation broth of Yarrowia lipolytica using aqueous two-phase system based on quaternary ammonium compounds

FS Buarque, A Carniel, BD Ribeiro… - Separation and …, 2023 - Elsevier
Aqueous two-phase systems (ATPS) are considered efficient and sustainable downstream
processing techniques in extracting and separating enzymes. In this work, we evaluated the …

[HTML][HTML] Advancements in aqueous two-phase systems for enzyme extraction, purification, and biotransformation

N Bekavac, M Benković, T Jurina, D Valinger… - Molecules, 2024 - mdpi.com
In recent years, the increasing need for energy conservation and environmental protection
has driven industries to explore more efficient and sustainable processes. Liquid–liquid …

Purification and characterization of catechol 1, 2-dioxygenase (EC 1.13. 11.1; catechol-oxygen 1, 2-oxidoreductase; C12O) using the local isolate of phenol-degrading …

HR Tawfeeq, SS Al-Jubori, AH Mussa - Folia Microbiologica, 2024 - Springer
The purpose of the present study was to purify and characterize the catechol 1, 2-
dioxygenase (EC 1.13. 11.1; catechol-oxygen 1, 2-oxidoreductase; C12O) enzyme from the …

Recent insight into the advances and prospects of microbial lipases and their potential applications in industry

A Eskandari, TC Leow, MBA Rahman… - International …, 2024 - Springer
Enzymes play a crucial role in various industrial sectors. These biocatalysts not only ensure
sustainability and safety but also enhance process efficiency through their unique specificity …

Salting-out as a Fast and Simple Method for First-Step Separation of Biobased 1, 3-Propanediol

V Liberato, FS Buarque, TF Ferreira… - Journal of Chemical & …, 2024 - ACS Publications
The cost of 1, 3-propanediol (1, 3-PDO) microbial production is very much dependent on the
separation of 1, 3-PDO from the fermentation broth. The use of byproducts as nutrient …

Cold-active lipase from Psychrobacter alimentarius ILMKVIT and its application in selective enrichment of ω-3 polyunsaturated fatty acids in flax seed oil

V Iswareya Lakshimi, M Kavitha - Bioprocess and Biosystems Engineering, 2024 - Springer
Lipases are one of the ubiquitous enzymes that belong to the hydrolases family and have a
wide variety of applications. Cold-active lipases are of major attraction as they can act in …

[HTML][HTML] Selection of Yarrowia lipolytica Lipases for Efficient Ester Synthesis or Hydrolysis

AH Souza, MFC Moura, RCB Franson, T Carvalho… - Reactions, 2024 - mdpi.com
The species Yarrowia lipolytica is an aerobic yeast that produces different lipase isoforms,
including extracellular, intracellular, and membrane-bound ones. The immobilization of …

[引用][C] STABILITY OF Yarrowia lipolytica IMUFRJ 50682 PROTEASES PARTIALLY PURIFIED BY AQUEOUS TWO-PHASE SYSTEMS

ASS CARVALHO, RM da SILVA, CN da SILVA…