In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Introduction: Nucleic-acid-based biopharmaceuticals enclose a remarkable potential for
treating debilitating or life-threatening diseases that currently remain incurable. This …

Plasmids are one of the most commonly used platforms for genetic engineering and
recombinant gene expression in bacteria. The range of available copy numbers for cloning …

Land application of animal manure, as an important agronomic practice, represents a
potential route to disseminate antibiotic resistance genes (ARGs) into the soil environment …

Bacterial cellulose (BC) exhibits unique properties such as high purity compared to plant-
based cellulose; however, commercial production of BC has remained a challenge, primarily …

The higher intracellular ATP levels of genome‐edited strains of P. putida that result from
deleting various energy‐consuming functions has been exploited for expanding the window …

Plasmid DNA (pDNA) has become very attractive as a biopharmaceutical, especially for
gene therapy and DNA vaccination. Currently, there are a few products licensed for …

Minicircular DNA (mcDNA) biopharmaceuticals have recently risen as a valuable alternative
for the development of a next generation of bioactive therapeutics because they are more …

BACKGROUND Recombinant protein excretion has become a mainstream strategy in
reducing downstream processing costs. However, recombinant protein excretion is often …

In order to provide sufficient pharmaceutical-grade plasmid DNA material, it is essential to
gain a comprehensive knowledge of the bioprocesses involved; so, the development of …