A snapshot of biomanufacturing and the need for enabling research infrastructure
E Asin-Garcia, JD Fawcett, C Batianis… - Trends in …, 2024 - cell.com
Biomanufacturing is crucial for the bioeconomy, with growing investment and attention from
industries and governments. Over recent decades numerous biotech companies have been …
industries and governments. Over recent decades numerous biotech companies have been …
Flux balance analysis and peptide mapping elucidate the impact of bioreactor pH on Chinese hamster ovary (CHO) cell metabolism and N-linked glycosylation in the …
Culture conditions have a profound impact on therapeutic protein production and
glycosylation, a critical therapeutic-quality attribute, especially for monoclonal antibodies …
glycosylation, a critical therapeutic-quality attribute, especially for monoclonal antibodies …
[HTML][HTML] Addressing amino acid‐derived inhibitory metabolites and enhancing CHO cell culture performance through DOE‐guided media modifications
P Ladiwala, VG Dhara, J Jenkins… - Biotechnology and …, 2023 - Wiley Online Library
Previously, we identified six inhibitory metabolites (IMs) accumulating in Chinese hamster
ovary (CHO) cultures using AMBIC 1.0 community reference medium that negatively …
ovary (CHO) cultures using AMBIC 1.0 community reference medium that negatively …
In-line HPLC-ICP-MS method for the rapid speciation and quantification of metal constituents in cell culture media
CJ Stouffer, SK Wysor, RK Marcus - Journal of Analytical Atomic …, 2024 - pubs.rsc.org
It is well established that the metal content in Chinese hamster ovary (CHO) cell culture
media (CCM) greatly affects process productivity and critical quality attributes (CQAs) …
media (CCM) greatly affects process productivity and critical quality attributes (CQAs) …
A Novel, Site‐Specific N‐Linked Glycosylation Model Provides Mechanistic Insights Into the Process‐Condition Dependent Distinct Fab and Fc Glycosylation of an …
The CHO VRC01 cell line produces an anti‐HIV IgG1 monoclonal antibody containing N‐
linked glycans on both the Fab (variable) and Fc (constant) regions. Site‐specific glycan …
linked glycans on both the Fab (variable) and Fc (constant) regions. Site‐specific glycan …
Model‐based control of titer and glycosylation in fed‐batch mAb production: Modeling and control system development
Therapeutic monoclonal antibodies (mAbs) are typically manufactured using mammalian
cell cultures in fed‐batch bioreactors, with increasing emphasis on meeting productivity and …
cell cultures in fed‐batch bioreactors, with increasing emphasis on meeting productivity and …
In-line buffer exchange in the coupling of Protein A chromatography with weak cation exchange chromatography for the determination of charge variants of …
SK Wysor, BF Synoground, SW Harcum… - … of Chromatography A, 2024 - Elsevier
Immunoglobulin G (IgG) is the most common monoclonal antibody (mAb) grown for
therapeutic applications. While IgG is often selectively isolated from cell lines using protein A …
therapeutic applications. While IgG is often selectively isolated from cell lines using protein A …
Small-scale perfusion mimic cultures in the ambr250 HT bioreactor system
Perfusion cell cultures generate higher viable cell densities (VCD) and volumetric
productivity compared to fed-batch cultures. However, due to the limited availability of small …
productivity compared to fed-batch cultures. However, due to the limited availability of small …
Prediction of CHO cell line stability using expression of DNA repair genes
LT Cordova, H Dahodwala, R Cooley… - Biotechnology …, 2024 - Wiley Online Library
Chinese hamster ovary (CHO) cells are essential to biopharmaceutical manufacturing and
production instability, the loss of productivity over time, is a long‐standing challenge in the …
production instability, the loss of productivity over time, is a long‐standing challenge in the …
Synergistic promotion of transient transgene expression in CHO cells by PDI/XBP-1s co-transfection and mild hypothermia
X Zhang, Y Wang, D Yi, C Zhang, B Ning, Y Fu… - Bioprocess and …, 2024 - Springer
Transient gene expression system is an important tool for rapid production of recombinant
proteins in Chinese hamster ovary (CHO) cells. However, their low productivity is the main …
proteins in Chinese hamster ovary (CHO) cells. However, their low productivity is the main …