Evolve and resequence (E&R) experiments use experimental evolution to adapt populations
to a novel environment, then next-generation sequencing to analyse genetic changes. They …

Self-cleaving ribozymes were discovered 30 years ago, but their biological distribution and
catalytic mechanisms are only beginning to be defined. Each ribozyme family is defined by a …

Ribozymes are noncoding RNAs that promote chemical transformations with rate
enhancements approaching those of protein enzymes. Although ribozymes are likely to …

Small endonucleolytic ribozymes promote the self-cleavage of their own phosphodiester
backbone at a specific linkage. The structures of and the reactions catalysed by members of …

The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-)
cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three …

Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by
internal phosphoester transfer. Comparative sequence analysis was used to identify …

We have determined the crystal structure of a pseudoknot (PK)-containing hammerhead
ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary …

RNAs are ideal for the design of gene switches that can monitor and program cellular
behavior because of their high modularity and predictable structure-function relationship …

HDV ribozymes catalyze their own scission from the transcript during rolling circle replication
of the hepatitis delta virus. In vitro selection of self-cleaving ribozymes from a human …

Although naturally occurring catalytic RNA molecules—ribozymes—have attracted a great
deal of research interest, very few have been identified in humans. Here, we developed a …