Analyses of nucleic acids (DNA and RNA) have become a staple tool for botanists to answer
questions across a wide variety of disciplines, ranging from population genetics to …

Premise The use of cetyltrimethylammonium bromide (CTAB) is an effective and
inexpensive method of extracting DNA from plants. The CTAB protocol is frequently modified …

Because of the heterogeneity among seedlings of outbreeding species, the use of seedling
tissues as a source of DNA is unsuitable for the genomic characterization of elite …

Next-generation sequencing demands high-quality nucleic acid, yet isolating DNA and RNA
is often challenging, particularly from plant tissues. Despite advances in developing various …

Premise Efficient protocols for extracting high‐molecular‐weight (HMW) DNA from ferns
facilitate the long‐read sequencing of their large and complex genomes. Here, we perform …

Low temperatures pose a critical challenge to aquaculture operations, as they can
significantly affect fish health and overall productivity. The study investigated the impact of …

Baccaurea ramiflora Lour., an evergreen tree of the Baccaurea genus of the Phyllanthaceae
family, is primarily distributed in South Asia, Southeast Asia, and southern China, including …

We report the optimization of a high-throughput, compliant DNA extraction method that uses
standard format 96-well plates and a commercial automated DNA purification system (ABI …

Premise Plants with stiff, leathery leaves pose a challenge for standard DNA extraction
protocols. These tissues are recalcitrant to mechanical disruption via TissueLyser (or …

Petunias are renowned ornamental species widely cultivated as pot plants for their aesthetic
appeal both indoors and outdoors. The preference for pot plants depends on their compact …