Microbial nattokinase: from synthesis to potential application

Y Sheng, J Yang, C Wang, X Sun, L Yan - Food & Function, 2023 - pubs.rsc.org
Nattokinase (NK) is an alkaline serine protease with strong thrombolytic activity produced by
Bacillus spp. or Pseudomonas spp. It is a potential therapeutic agent for thrombotic diseases …

Research progress on the fibrinolytic enzymes produced from traditional fermented foods

P Wang, C Peng, X Xie, X Deng… - Food Science & …, 2023 - Wiley Online Library
Cardiovascular diseases (CVDs) are a global health problem and leading cause of death
worldwide. Thrombus formation, one of the CVDs, is essentially the formation of fibrin clots …

Improvement of the fibrinolytic activity, acid resistance and thermostability of nattokinase by surface charge engineering

Y Li, X Tang, L Chen, A Ma, W Zhu, W Huang… - International Journal of …, 2023 - Elsevier
Nattokinase is a promising thrombolytic drug due to its powerful fibrinolytic effect and few
side effects. However, the low fibrinolytic activity and stability of nattokinase have limited its …

Gene expression and molecular characterization of recombinant subtilisin from Bacillus subtilis with antibacterial, antioxidant and anticancer properties

SS Shettar, ZK Bagewadi, DA Yaraguppi, S Das… - International Journal of …, 2023 - Elsevier
This study investigated the multifunctional attributes such as, antibacterial, antioxidant and
anticancer potential of recombinant subtilisin. A codon-optimized subtilisin gene was …

[HTML][HTML] Optimization of subtilisin production from Bacillus subtilis strain ZK3 and biological and molecular characterization of synthesized subtilisin capped …

SS Shettar, ZK Bagewadi, HN Kolvekar… - Saudi Journal of …, 2023 - Elsevier
The increase and dissemination of multi-drug resistant bacteria have presented a major
healthcare challenge, making bacterial infections a significant concern. The present …

Combined Computer-Aided Predictors to Improve the Thermostability of Nattokinase

Y Li, L Chen, X Tang, W Zhu, A Ma, C Shi, J Li - Foods, 2023 - mdpi.com
Food-derived nattokinase has strong thrombolytic activity and few side effects. In the field of
medicine, nattokinase has been developed as an adjuvant drug for the treatment of …

[HTML][HTML] Genome Analysis and Optimization of Caproic Acid Production of Clostridium butyricum GD1-1 Isolated from the Pit Mud of Nongxiangxing Baijiu

M Li, T Li, J Zheng, Z Qiao, K Zhang… - … of Microbiology and …, 2023 - ncbi.nlm.nih.gov
Caproic acid is a precursor substance for the synthesis of ethyl caproate, the main flavor
substance of nongxiangxing baijiu liquor. In this study, Clostridium butyricum GD1-1, a strain …

Automated microscopic measurement of fibrinaloid microclots and their degradation by nattokinase, the main natto protease

JM Grixti, CW Theron, JE Salcedo-Sora, E Pretorius… - bioRxiv, 2024 - biorxiv.org
Nattokinase, from the Japanese fermented food natto, is a protease with fibrinolytic activity
that can thus degrade conventional blood clots. In some cases, however, including in Long …

Phenotypic and genomic insights into mutant with high nattokinase-producing activity induced by carbon ion beam irradiation of Bacillus subtilis

Y Sheng, S Zhang, X Li, S Wang, T Liu, C Wang… - International Journal of …, 2024 - Elsevier
Nattokinase (NK) is found in fermented foods and has high fibrinolytic activity, which makes
it promising for biological applications. In this study, a mutant strain (Bacillus subtilis ZT-S1 …

[PDF][PDF] Proteolytic targets for SARS-CoV-2 spike protein degradation: Hope for systemic detoxification

AM Kyriakopoulos, S Seneff - Journal of American Physicians and …, 2023 - researchgate.net
The SARS-CoV-2 spike protein is a long-lasting foreign pathogenic protein found in cells
and tissues after COVID-19 respiratory illness and COVID-19 vaccination. The spike protein …