Detection technologies in proteome analysis

WF Patton - Journal of Chromatography B, 2002 - Elsevier
Common strategies employed for general protein detection include organic dye, silver stain,
radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass …

Host cell protein testing by ELISAs and the use of orthogonal methods

J Zhu‐Shimoni, C Yu, J Nishihara… - Biotechnology and …, 2014 - Wiley Online Library
Host cell proteins (HCPs) are among the process‐related impurities monitored during
recombinant protein pharmaceutical process development. The challenges of HCP …

Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone

L Wilson-Fritch, A Burkart, G Bell… - … and cellular biology, 2003 - Taylor & Francis
White adipose tissue is an important endocrine organ involved in the control of whole-body
metabolism, insulin sensitivity, and food intake. To better understand these functions, 3T3-L1 …

In-line probing analysis of riboswitches

EE Regulski, RR Breaker - Post-transcriptional gene regulation, 2008 - Springer
Riboswitches are intricate, metabolite-binding RNA structures found in the non-coding
regions of mRNA. Allosteric changes in the riboswitch that are induced by metabolite …

Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology

TH Steinberg, BJ Agnew, KR Gee… - PROTEOMICS …, 2003 - Wiley Online Library
Systematic parallel analysis of the phosphorylation status of networks of interacting proteins
involved in the regulatory circuitry of cells and tissues is certain to drive research in the post …

Quantitation, visualization, and monitoring of conformational transitions of human serum albumin by a tetraphenylethene derivative with aggregation-induced emission …

Y Hong, C Feng, Y Yu, J Liu, JWY Lam… - Analytical …, 2010 - ACS Publications
Human serum albumin (HSA) is a major protein component of blood plasma, and its assay is
of obvious value to biological research. We, herein, present a readily accessible fluorescent …

Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation

KS Lilley, A Razzaq, P Dupree - Current opinion in chemical biology, 2002 - Elsevier
A strength of two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is its ability to
resolve and investigate the abundance of several thousand proteins in a single sample. This …

[36] Gel-staining techniques

CR Merril - Methods in enzymology, 1990 - Elsevier
Publisher Summary Naturally colored proteins, such as myoglobin, hemoglobin, ferritin, and
cytochrome c may be directly observed in gels illuminated with light in the visual spectrum …

Chemical strategies for activity‐based proteomics

AE Speers, BF Cravatt - ChemBioChem, 2004 - Wiley Online Library
The assignment of molecular and cellular functions to the numerous protein products
encoded by prokaryotic and eukaryotic genomes presents a major challenge for the field of …

Protein stains for proteomic applications: Which, when, why?

I Miller, J Crawford, E Gianazza - Proteomics, 2006 - Wiley Online Library
This review recollects literature data on sensitivity and dynamic range for the most
commonly used colorimetric and fluorescent dyes for general protein staining, and …