Highlights•Compares and contrasts new affinity methods to dominant conventional
methods.•Explains how the popular His tag, despite its age, still dominates this …

The purification of recombinant proteins by affinity chromatography is one of the most
efficient strategies due to the high recovery yields and purity achieved. However, this is …

Inspired by the remarkable ability of natural protein switches to sense and respond to a wide
range of environmental queues, here we report a strategy to engineer synthetic protein …

Outer membrane vesicles (OMVs) are nanoscale spherical vesicles released from Gram‐
negative bacteria. The lipid bilayer membrane structure of OMVs consists of similar …

Currently, there are over 100 antibody-based therapeutics on the market for the treatment of
various diseases. The increasing importance of antibody treatment is further highlighted by …

The affinity tags are unique proteins/peptides that are attached at the N-or C-terminus of the
recombinant proteins. These tags help in protein purification. Additionally, some affinity tags …

We report here a new strategy to enable fast, covalent, and site-directed functionalization of
protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging …

Downstream processing is considered one of the most challenging phases of industrial
manufacturing of therapeutic proteins, accounting for a large portion of the total production …

Outer membrane vesicles (OMVs) are proteoliposomes derived from the outer membrane
and periplasmic space of many Gram-negative bacteria including E. coli as part of their …

The separation of antibodies from complex mixtures can be achieved using
chromatographic or non-chromatographic techniques. The purification of antibodies using …