Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications
BP Tracy, SW Jones, AG Fast, DC Indurthi… - Current opinion in …, 2012 - Elsevier
Clostridia are anaerobic Firmicutes producing a large array of metabolites by utilizing simple
and complex carbohydrates, such as cellulose, as well as CO2/H2 or CO. Their exceptional …
and complex carbohydrates, such as cellulose, as well as CO2/H2 or CO. Their exceptional …
Type IV pili in Gram-positive bacteria
S Melville, L Craig - Microbiology and molecular biology reviews, 2013 - Am Soc Microbiol
SUMMARY Type IV pili (T4P) are surface-exposed fibers that mediate many functions in
bacteria, including locomotion, adherence to host cells, DNA uptake (competence), and …
bacteria, including locomotion, adherence to host cells, DNA uptake (competence), and …
Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators
Eggerthella lenta is a prevalent human gut Actinobacterium implicated in drug, dietary
phytochemical, and bile acid metabolism and associated with multiple human diseases. No …
phytochemical, and bile acid metabolism and associated with multiple human diseases. No …
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …
Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii …
CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
Recent Developments of the Synthetic Biology Toolkit for Clostridium
The Clostridium genus is a large, diverse group consisting of Gram-positive, spore-forming,
obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well …
obligate anaerobic firmicutes. Among this group are historically notorious pathogens as well …
Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium …
M Flaiz, G Ludwig, FR Bengelsdorf, P Dürre - Biotechnology for Biofuels, 2021 - Springer
Background The interest in using methanol as a substrate to cultivate acetogens increased
in recent years since it can be sustainably produced from syngas and has the additional …
in recent years since it can be sustainably produced from syngas and has the additional …
Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms
Unabated mining and utilisation of petroleum and petroleum resources and their conversion
to essential fuels and chemicals have drastic environmental consequences, contributing to …
to essential fuels and chemicals have drastic environmental consequences, contributing to …
Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii
The development of tools for genetic manipulation of Clostridium ljungdahlii has increased
its attractiveness as a chassis for autotrophic production of organic commodities and …
its attractiveness as a chassis for autotrophic production of organic commodities and …
[HTML][HTML] A roadmap for gene system development in Clostridium
Clostridium species are both heroes and villains. Some cause serious human and animal
diseases, those present in the gut microbiota generally contribute to health and wellbeing …
diseases, those present in the gut microbiota generally contribute to health and wellbeing …