In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants

B Ahmadi, H Ebrahimzadeh - Plant cell reports, 2020 - Springer
Androgenesis has become the most frequently chosen method of doubled haploid (DH)
production in major crops. Theoretically, plantlets derived from in vitro cultured microspore …

Androgenesis in solanaceae

JM Seguí-Simarro - In vitro embryogenesis in higher plants, 2016 - Springer
The Solanaceae is one of the most important families for global agriculture. Among the
different solanaceous species, tobacco (Nicotiana tabacum), potato (Solanum tuberosum) …

Comparison of six different methods to calculate cell densities

C Camacho-Fernández, D Hervás, A Rivas-Sendra… - Plant Methods, 2018 - Springer
Background For in vitro culture of plant and animal cells, one of the critical steps is to adjust
the initial cell density. A typical example of this is isolated microspore culture, where specific …

Stress treatments and in vitro culture conditions influence microspore embryogenesis and growth of callus from anther walls of sweet pepper (Capsicum annuum L.)

V Parra-Vega, B Renau-Morata, A Sifres… - Plant Cell, Tissue and …, 2013 - Springer
Production of doubled haploids (DHs) is a convenient tool to obtain pure lines for breeding
purposes. Until now, the easiest and most useful approach to obtain pepper DHs is via …

Enhancement of microspore embryogenesis induction and plantlet regeneration of sweet pepper (Capsicum annuum L.) using putrescine and ascorbic acid

AA Heidari-Zefreh, ME Shariatpanahi, A Mousavi… - Protoplasma, 2019 - Springer
Production of doubled haploid (DH) plants is an efficient tool in genetic and plant breeding
programs; however, sweet pepper (Capsicum annuum L.) is recalcitrant to microspore …

Study of in vitro anther culture in selected genotypes of genus Capsicum

D Olszewska, A Kisiala… - Turkish Journal of …, 2014 - journals.tubitak.gov.tr
The combined effect of anther incubation time on CP induction medium (12, 14, and 16
days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the …

Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.)

E Ari, T Yildirim, N Mutlu, S Büyükalaca… - Turkish Journal of …, 2016 - journals.tubitak.gov.tr
Doubled haploid (DH) plant production plays an important role in pepper (Capsicum
annuum L.) breeding for development of new varieties. However, information is lacking for …

Species with haploid or doubled haploid protocols

JM Seguí-Simarro, JB Moreno, MG Fernández… - … Technology: Volume 1 …, 2021 - Springer
In this chapter, we present a list of species (and few interspecific hybrids) where haploids
and/or doubled haploids have been published, including the method by which they were …

[HTML][HTML] Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L.)

Y Cheng, R Ma, Y Jiao, N Qiao, T Li - South African Journal of Botany, 2013 - Elsevier
The production of double haploids through androgenesis is used by breeders to produce
homozygous lines in a single generation. Androgenesis can be achieved by isolated …

Current status of haploidization in cool-season grain legume crop species

W Skrzypkowski, A Kiełkowska - Agriculture, 2024 - mdpi.com
Doubled haploid technology is, so far, the fastest route to induce a true homozygous state in
plants. True homozygous plants are particularly important for breeders, as they can facilitate …