Thermal stability enhancement: Fundamental concepts of protein engineering strategies to manipulate the flexible structure

M Rahban, S Zolghadri, N Salehi, F Ahmad… - International journal of …, 2022 - Elsevier
Increasing the temperature by just a few degrees may lead to structural perturbation or
unfolding of the protein and consequent loss of function. The concepts of flexibility and …

Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …

The state-of-the-art strategies of protein engineering for enzyme stabilization

Q Liu, G Xun, Y Feng - Biotechnology advances, 2019 - Elsevier
Enzymes generated by natural recruitment and protein engineering have greatly contribute
in various sets of applications. However, their insufficient stability is a bottleneck that limit the …

Post-translational modifications of protein backbones: unique functions, mechanisms, and challenges

MM Müller - Biochemistry, 2018 - ACS Publications
Post-translational modifications (PTMs) dramatically enhance the capabilities of proteins.
They introduce new functionalities and dynamically control protein activity by modulating …

Machine learning applied to predicting microorganism growth temperatures and enzyme catalytic optima

G Li, KS Rabe, J Nielsen, MKM Engqvist - ACS synthetic biology, 2019 - ACS Publications
Enzymes that catalyze chemical reactions at high temperatures are used for industrial
biocatalysis, applications in molecular biology, and as highly evolvable starting points for …

Sea cucumber derived type I collagen: A comprehensive review

TRL Senadheera, D Dave, F Shahidi - Marine Drugs, 2020 - mdpi.com
Collagen is the major fibrillar protein in most living organisms. Among the different types of
collagen, type I collagen is the most abundant one in tissues of marine invertebrates. Due to …

Engineering thermostable microbial xylanases toward its industrial applications

V Kumar, AK Dangi, P Shukla - Molecular biotechnology, 2018 - Springer
Xylanases are one of the important hydrolytic enzymes which hydrolyze the β-1, 4 xylosidic
linkage of the backbone of the xylan polymeric chain which consists of xylose subunits …

A statistical analysis of the sequence and structure of thermophilic and non-thermophilic proteins

Z Ahmed, H Zulfiqar, L Tang, H Lin - International Journal of Molecular …, 2022 - mdpi.com
Thermophilic proteins have various practical applications in theoretical research and in
industry. In recent years, the demand for thermophilic proteins on an industrial scale has …

Community-level respiration of prokaryotic microbes may rise with global warming

TP Smith, TJH Thomas, B García-Carreras… - Nature …, 2019 - nature.com
Understanding how the metabolic rates of prokaryotes respond to temperature is
fundamental to our understanding of how ecosystem functioning will be altered by climate …

A critical review on the environmental application of lipopeptide micelles

Z Zhu, B Zhang, Q Cai, Y Cao, J Ling, K Lee… - Bioresource …, 2021 - Elsevier
The importance of lipopeptide micelles in environmental applications has been highlighted.
These vessels exhibit various sizes, shapes, and surface properties under different …