The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Highlights•SICLOPPS allows the rapid generation of cyclic peptide libraries in cells.•A
variety of cyclic peptide ring sizes may be generated with SICLOPPS.•SICLOPPS libraries …

A variety of chemicals have been produced through metabolic engineering approaches, and
enhancing biosynthesis performance can be achieved by using enzymes with high catalytic …

Cyclic peptides are an emerging class of molecular therapeutics that are increasingly
viewed as ideal backbones for modulation of protein–protein interactions. A split‐intein …

The virtual screening of a library of xanthone derivatives led us to the identification of
potential novel MDM2 ligands. The activity of these compounds as inhibitors of p53–MDM2 …

The development of protein–protein interaction (PPI) inhibitors with therapeutic value is of
increasing importance as the first clinical agent has now been approved, but PPIs remain …

DNA-encoded libraries hold great potential for discovering small, cyclized peptides with
drug potential. Split-intein circular ligation of peptides and proteins (SICLOPPS) is a well …

Abstract Development of endoproteases, programmed to promote degradation of peptides
or proteins responsible for pathogenic states, represents an attractive therapeutic strategy …

Alterations of p53 signalling pathway is the most frequent event in human cancers. About
50% of these, albeit showing wild-type p53, have flaws in the control mechanisms of p53 …

Optimization of chemical reactions, discovery of optimal substrates and determination of
substrate scope involve exhaustive screening of conditions as well as measurements of …