Cell culture in microfluidic droplets

S Sart, G Ronteix, S Jain, G Amselem… - Chemical …, 2022 - ACS Publications
Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …

Bioengineering approaches for the advanced organoid research

SA Yi, Y Zhang, C Rathnam, T Pongkulapa… - Advanced …, 2021 - Wiley Online Library
Recent advances in 3D cell culture technology have enabled scientists to generate stem cell
derived organoids that recapitulate the structural and functional characteristics of native …

Recent methods of droplet microfluidics and their applications in spheroids and organoids

Y Wang, M Liu, Y Zhang, H Liu, L Han - Lab on a Chip, 2023 - pubs.rsc.org
Droplet microfluidic techniques have long been known as a high-throughput approach for
cell manipulation. The capacity to compartmentalize cells into picolitre droplets in …

Microfluidic organoids-on-a-chip: Quantum leap in cancer research

F Duzagac, G Saorin, L Memeo, V Canzonieri… - Cancers, 2021 - mdpi.com
Simple Summary Organoids, also known as self-organized 3D organ-like clusters, represent
almost a physiological system for studying cells, stem cells, tissues, and diseases, especially …

Individual control and quantification of 3D spheroids in a high-density microfluidic droplet array

RFX Tomasi, S Sart, T Champetier, CN Baroud - Cell reports, 2020 - cell.com
As three-dimensional cell culture formats gain in popularity, there emerges a need for tools
that produce vast amounts of data on individual cells within the spheroids or organoids …

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

P Zhang, N Shao, L Qin - Advanced Materials, 2021 - Wiley Online Library
Cell‐based living materials, including single cells, cell‐laden fibers, cell sheets, organoids,
and organs, have attracted intensive interests owing to their widespread applications in …

Three-dimensional Spheroid Culture Enhances Multipotent Differentiation and Stemness Capacities of Human Dental Pulp‐derived Mesenchymal Stem Cells by …

YH Chan, YC Lee, CY Hung, PJ Yang, PC Lai… - Stem Cell Reviews and …, 2021 - Springer
Abstract Background Three-dimensional (3D) culture of mesenchymal stem cells has
become an important research and development topic. However, comprehensive analysis of …

High resolution microfluidic assay and probabilistic modeling reveal cooperation between T cells in tumor killing

G Ronteix, S Jain, C Angely, M Cazaux… - Nature …, 2022 - nature.com
Cytotoxic T cells are important components of natural anti-tumor immunity and are
harnessed in tumor immunotherapies. Immune responses to tumors and immune therapy …

[HTML][HTML] Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures

JC Cossec, T Traboulsi, S Sart, Y Loe-Mie… - Cell Reports, 2023 - cell.com
Recent advances in synthetic embryology have opened new avenues for understanding the
complex events controlling mammalian peri-implantation development. Here, we show that …

[HTML][HTML] Combinatorial drug screening on 3D Ewing sarcoma spheroids using droplet-based microfluidics

R Fevre, G Mary, N Vertti-Quintero, A Durand… - Iscience, 2023 - cell.com
Culturing and screening cells in microfluidics, particularly in three-dimensional formats, has
the potential to impact diverse areas from fundamental biology to cancer precision medicine …