In situ cryo-electron tomography: a post-reductionist approach to structural biology
Cryo-electron tomography is a powerful technique that can faithfully image the native
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo …
cellular environment at nanometer resolution. Unlike many other imaging approaches, cryo …
Cellular electron cryotomography: toward structural biology in situ
CM Oikonomou, GJ Jensen - Annual review of biochemistry, 2017 - annualreviews.org
Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …
Cryo‐fluorescence microscopy facilitates correlations between light and cryo‐electron microscopy and reduces the rate of photobleaching
CL Schwartz, VI Sarbash, FI Ataullakhanov… - Journal of …, 2007 - Wiley Online Library
Fluorescence light microscopy (LM) has many advantages for the study of cell organization.
Specimen preparation is easy and relatively inexpensive, and the use of appropriate tags …
Specimen preparation is easy and relatively inexpensive, and the use of appropriate tags …
Electron tomography of cells
The electron microscope has contributed deep insights into biological structure since its
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …
Cryo‐FIB‐lift‐out: practically impossible to practical reality
CD Parmenter, ZA Nizamudeen - Journal of Microscopy, 2021 - Wiley Online Library
In this paper, we explore the development of the Cryo‐Lift‐Out (cryo‐LO) technique as
preparation tool for cryogenic transmission electron microscopy (cryo‐TEM). What started in …
preparation tool for cryogenic transmission electron microscopy (cryo‐TEM). What started in …
A new view into prokaryotic cell biology from electron cryotomography
Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …
Correlative light and electron microscopy methods for the study of virus–cell interactions
Electron microscopy (EM) is an invaluable tool to study the interactions of viruses with cells,
and the ultrastructural changes induced in host cells by virus infection. Light microscopy …
and the ultrastructural changes induced in host cells by virus infection. Light microscopy …
A fractal model for nuclear organization: current evidence and biological implications
Chromatin is a multiscale structure on which transcription, replication, recombination and
repair of the genome occur. To fully understand any of these processes at the molecular …
repair of the genome occur. To fully understand any of these processes at the molecular …
Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells
LF van Driel, JA Valentijn, KM Valentijn… - European journal of cell …, 2009 - Elsevier
Cryo-electron tomography (cryo-ET) allows for the visualization of biological material in a
close-to-native state, in three dimensions and with nanometer scale resolution. However …
close-to-native state, in three dimensions and with nanometer scale resolution. However …
How does the spacer length of cationic gemini lipids influence the lipoplex formation with plasmid DNA? Physicochemical and biochemical characterizations and their …
Lipoplexes formed by the pEGFP-C3 plasmid DNA (pDNA) and lipid mixtures containing
cationic gemini surfactant of the 1, 2-bis (hexadecyl dimethyl ammonium) alkanes family …
cationic gemini surfactant of the 1, 2-bis (hexadecyl dimethyl ammonium) alkanes family …