Biological nanopore approach for single‐molecule protein sequencing

ZL Hu, MZ Huo, YL Ying, YT Long - Angewandte Chemie, 2021 - Wiley Online Library
Proteins are responsible for the occurrence and treatment of many diseases, and therefore
protein sequencing will revolutionize proteomics and clinical diagnostics. Biological …

Engineering biological nanopore approaches toward protein sequencing

X Wei, T Penkauskas, JE Reiner, C Kennard… - ACS …, 2023 - ACS Publications
Biotechnological innovations have vastly improved the capacity to perform large-scale
protein studies, while the methods we have for identifying and quantifying individual proteins …

Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force

A Sauciuc, B Morozzo della Rocca, MJ Tadema… - Nature …, 2023 - nature.com
Nanopores have recently been used to identify and fingerprint proteins. However, because
proteins, unlike DNA, do not have a uniform charge, the electrophoretic force cannot in …

[HTML][HTML] Enzyme-less nanopore detection of post-translational modifications within long polypeptides

P Martin-Baniandres, WH Lan, S Board… - Nature …, 2023 - nature.com
Means to analyse cellular proteins and their millions of variants at the single-molecule level
would uncover substantial information previously unknown to biology. Nanopore …

[HTML][HTML] FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution

G Huang, A Voet, G Maglia - Nature communications, 2019 - nature.com
A high throughput single-molecule method for identifying peptides and sequencing proteins
based on nanopores could reduce costs and increase speeds of sequencing, allow the …

Single-molecule protein sensing in a nanopore: a tutorial

N Varongchayakul, J Song, A Meller… - Chemical Society …, 2018 - pubs.rsc.org
Proteins are the structural elements and machinery of cells responsible for a functioning
biological architecture and homeostasis. Advances in nanotechnology are catalyzing key …

Mapping the acetylamino and carboxyl groups on glycans by engineered α-hemolysin nanopores

B Xia, J Fang, S Ma, M Ma, G Yao, T Li… - Journal of the …, 2023 - ACS Publications
Glycan is a crucial class of biological macromolecules with important biological functions.
Functional groups determine the chemical properties of glycans, which further affect their …

[HTML][HTML] Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores

G Huang, K Willems, M Soskine, C Wloka… - Nature …, 2017 - nature.com
Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and
long read-length DNA sequencing applications. The analysis and sequencing of proteins …

Nanopore-based single-biomolecule interfaces: from information to knowledge

YL Ying, YT Long - Journal of the American Chemical Society, 2019 - ACS Publications
Single-molecule measurements have greatly enhanced our understanding of living systems.
Biological systems offer nanopores, a sub-class of membrane proteins, the well-defined …

Overview of the materials design and sensing strategies of nanopore devices

L Liang, F Qin, S Wang, J Wu, R Li, Z Wang… - Coordination Chemistry …, 2023 - Elsevier
Nanopore platform holds a great fascination that attracts increasing interests in research and
leads the development of various domains. Herein we first briefly introduce the …