290 MURRAY & SZOSTAK was present in the parental cell. Chromosome segregation is an
extremely accurate process: Chromosome loss in mitosis in yeast occurs once in every 105 …

INTRODUCTION of important advantages for the study of eucaryotic DNA replication and
chromosome segregation. In contrast to Eucaryotic chromosomes, each of which consists of …

The methods described in this chapter permit the manipulation of virtually any cloned yeast
chromosomal sequence by virtue of the fact that DNA transformed into yeast integrates into …

We have combined long synthetic arrays of alpha satellite DNA with telomeric DNA and
genomic DNA to generate artificial chromosomes in human HT1080 cells. The resulting …

The MIF2 gene of Saccharomyces cerevisiae has been implicated in mitosis. Here we
provide genetic evidence that MIF2 encodes a centromere protein. Specifically, we found …

A set of genomic plasmid banks was constructed using the centromere-containing yeast
shuttle vector YCp50. The centromere-containing vector is useful for the isolation of genes …

A colony color assay that measures chromosome stability is described and is used to study
several parameters affecting the mitotic maintenance of yeast chromosomes, including ARS …

We have screened for the presence of two centromere autoantigens, CENP-B (80 kDa) and
CENP-C (140 kDa) at the inactive centromere of a naturally occurring stable dicentric …

A conditional centromere was constructed in Saccharomyces cerevisiae by placing the
centromere of chromosome III immediately downstream from the inducible GAL1 promoter …

The hypothesis that DNA topoisomerase II facilitates the separation of replicated sister
chromatids was tested by examining the consequences of chromosome segregation in the …