Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Decoding the spermatogenesis program: new insights from transcriptomic analyses

M Rabbani, X Zheng, GL Manske… - Annual review of …, 2022 - annualreviews.org
Spermatogenesis is a complex differentiation process coordinated spatiotemporally across
and along seminiferous tubules. Cellular heterogeneity has made it challenging to obtain …

The molecular evolution of spermatogenesis across mammals

F Murat, N Mbengue, SB Winge, T Trefzer, E Leushkin… - Nature, 2023 - nature.com
The testis produces gametes through spermatogenesis and evolves rapidly at both the
morphological and molecular level in mammals,,,,–, probably owing to the evolutionary …

Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila

CN McLaughlin, M Brbić, Q Xie, T Li, F Horns… - Elife, 2021 - elifesciences.org
Recognition of environmental cues is essential for the survival of all organisms.
Transcriptional changes occur to enable the generation and function of the neural circuits …

Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys

K Mizuta, Y Katou, B Nakakita, A Kishine… - The EMBO …, 2022 - embopress.org
In vitro oogenesis is key to elucidating the mechanism of human female germ‐cell
development and its anomalies. Accordingly, pluripotent stem cells have been induced into …

A reference single-cell regulomic and transcriptomic map of cynomolgus monkeys

J Qu, F Yang, T Zhu, Y Wang, W Fang, Y Ding… - Nature …, 2022 - nature.com
Non-human primates are attractive laboratory animal models that accurately reflect both
developmental and pathological features of humans. Here we present a compendium of cell …

Single‐cell RNA‐sequencing provides insight into skeletal muscle evolution during the selection of muscle characteristics

D Xu, B Wan, K Qiu, Y Wang, X Zhang, N Jiao… - Advanced …, 2023 - Wiley Online Library
Skeletal muscle comprises a large, heterogeneous assortment of cell populations that
interact to maintain muscle homeostasis, but little is known about the mechanism that …

Understanding testicular single cell transcriptional atlas: from developmental complications to male infertility

MB Tirumalasetty, I Bhattacharya… - Frontiers in …, 2024 - frontiersin.org
Spermatogenesis is a multi-step biological process where mitotically active diploid (2n)
spermatogonia differentiate into haploid (n) spermatozoa via regulated meiotic …

Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis

L Zhang, F Li, P Lei, M Guo, R Liu, L Wang, T Yu… - Journal of Animal …, 2021 - Springer
Background Spermatogenesis is the process by which male gametes are formed from
spermatogonial stem cells and it is essential for the reliable transmission of genetic …

Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with …

HY Wang, X Liu, JY Chen, Y Huang, Y Lu, F Tan… - Science China Life …, 2023 - Springer
Female-to-male sex reversals (pseudomales) are common in lower vertebrates and have
been found in natural populations, which is a concern under rapid changes in environmental …