Next-generation sequencing: insights to advance clinical investigations of the microbiome

CR Wensel, JL Pluznick, SL Salzberg… - The Journal of clinical …, 2022 - Am Soc Clin Investig
Next-generation sequencing (NGS) technology has advanced our understanding of the
human microbiome by allowing for the discovery and characterization of unculturable …

Microbial community-driven etiopathogenesis of peri-implantitis

GN Belibasakis, D Manoil - Journal of dental research, 2021 - journals.sagepub.com
Osseointegrated dental implants are a revolutionary tool in the armament of reconstructive
dentistry, employed to replace missing teeth and restore masticatory, occlusal, and esthetic …

Primer, pipelines, parameters: issues in 16S rRNA gene sequencing

I Abellan-Schneyder, MS Matchado, S Reitmeier… - Msphere, 2021 - Am Soc Microbiol
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics

L Xu, Z Dong, D Chiniquy, G Pierroz, S Deng… - Nature …, 2021 - nature.com
Recent studies have demonstrated that drought leads to dramatic, highly conserved shifts in
the root microbiome. At present, the molecular mechanisms underlying these responses …

Identification of microbial markers across populations in early detection of colorectal cancer

Y Wu, N Jiao, R Zhu, Y Zhang, D Wu, AJ Wang… - Nature …, 2021 - nature.com
Associations between gut microbiota and colorectal cancer (CRC) have been widely
investigated. However, the replicable markers for early-stage adenoma diagnosis across …

The gut microbiome in hypertension: recent advances and future perspectives

EG Avery, H Bartolomaeus, A Maifeld, L Marko… - Circulation …, 2021 - Am Heart Assoc
The pathogenesis of hypertension is known to involve a diverse range of contributing factors
including genetic, environmental, hormonal, hemodynamic and inflammatory forces, to …

Comparative analysis of 16S rRNA gene and metagenome sequencing in pediatric gut microbiomes

D Peterson, KS Bonham, S Rowland… - Frontiers in …, 2021 - frontiersin.org
The colonization of the human gut microbiome begins at birth, and over time, these microbial
communities become increasingly complex. Most of what we currently know about the …

A guide to human microbiome research: study design, sample collection, and bioinformatics analysis

XB Qian, T Chen, YP Xu, L Chen, FX Sun… - Chinese Medical …, 2020 - mednexus.org
The purpose of this review is to provide medical researchers, especially those without a
bioinformatics background, with an easy-to-understand summary of the concepts and …

The gut–eye axis: lessons learned from murine models

JL Floyd, MB Grant - Ophthalmology and therapy, 2020 - Springer
A healthy gut microbiota is essential in maintaining the human body in a homeostatic state
by its functions in digestion and immune tolerance. Under states of aberrant microbial …

Ocular microbiota and intraocular inflammation

JJ Li, S Yi, L Wei - Frontiers in Immunology, 2020 - frontiersin.org
The term ocular microbiota refers to all types of commensal and pathogenic microorganisms
present on or in the eye. The ocular surface is continuously exposed to the environment and …