An overview of technical considerations for Western blotting applications to physiological research

JJ Bass, DJ Wilkinson, D Rankin… - … journal of medicine …, 2017 - Wiley Online Library
The applications of Western/immunoblotting (WB) techniques have reached multiple layers
of the scientific community and are now considered routine procedures in the field of …

[HTML][HTML] A systematic approach to quantitative Western blot analysis

L Pillai-Kastoori, AR Schutz-Geschwender… - Analytical …, 2020 - Elsevier
Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

The design of a quantitative western blot experiment

SC Taylor, A Posch - BioMed research international, 2014 - Wiley Online Library
Western blotting is a technique that has been in practice for more than three decades that
began as a means of detecting a protein target in a complex sample. Although there have …

The necessity of and strategies for improving confidence in the accuracy of western blots

R Ghosh, JE Gilda, AV Gomes - Expert review of proteomics, 2014 - Taylor & Francis
Western blotting is one of the most commonly used laboratory techniques for identifying
proteins and semi-quantifying protein amounts; however, several recent findings suggest …

A defined methodology for reliable quantification of Western blot data

SC Taylor, T Berkelman, G Yadav, M Hammond - Molecular biotechnology, 2013 - Springer
Chemiluminescent western blotting has been in common practice for over three decades,
but its use as a quantitative method for measuring the relative expression of the target …

Superior mitochondrial adaptations in human skeletal muscle after interval compared to continuous single‐leg cycling matched for total work

MJ MacInnis, E Zacharewicz, BJ Martin… - The Journal of …, 2017 - Wiley Online Library
Key points A classic unresolved issue in human integrative physiology involves the role of
exercise intensity, duration and volume in regulating skeletal muscle adaptations to training …

[HTML][HTML] Proteomes are of proteoforms: embracing the complexity

K Carbonara, M Andonovski, JR Coorssen - Proteomes, 2021 - mdpi.com
Proteomes are complex—much more so than genomes or transcriptomes. Thus, simplifying
their analysis does not simplify the issue. Proteomes are of proteoforms, not canonical …

Important considerations for protein analyses using antibody based techniques: down‐sizing Western blotting up‐sizes outcomes

RM Murphy, GD Lamb - The Journal of physiology, 2013 - Wiley Online Library
Western blotting has been used for protein analyses in a wide range of tissue samples for>
30 years. Fundamental to Western blotting success are a number of important …

Omega‐3 fatty acid supplementation attenuates skeletal muscle disuse atrophy during two weeks of unilateral leg immobilization in healthy young women

C Mcglory, SHM Gorissen, M Kamal… - The FASEB …, 2019 - Wiley Online Library
ABSTRACT Omega‐3 (n‐3) fatty acid supplementation enhances muscle protein synthesis
and muscle size. Whether n‐3 fatty acid supplementation attenuates human muscle disuse …

Multiplex PCR in species authentication: probability and prospects—a review

ME Ali, MA Razzak, SBA Hamid - Food Analytical Methods, 2014 - Springer
Food forgery is one of the most articulated socio-economic concerns which contributed to
increase people's awareness on what they eat and how and where it is produced …