In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Electroporation in food processing and biorefinery

S Mahnič-Kalamiza, E Vorobiev, D Miklavčič - The Journal of membrane …, 2014 - Springer
Electroporation is a method of treatment of plant tissue that due to its nonthermal nature
enables preservation of the natural quality, colour and vitamin composition of food products …

Design and optimization of a light-emitting diode projection micro-stereolithography three-dimensional manufacturing system

X Zheng, J Deotte, MP Alonso, GR Farquar… - Review of Scientific …, 2012 - pubs.aip.org
The rapid manufacture of complex three-dimensional micro-scale components has eluded
researchers for decades. Several additive manufacturing options have been limited by either …

The effects of electroporation buffer composition on cell viability and electro-transfection efficiency

JJ Sherba, S Hogquist, H Lin, JW Shan, DI Shreiber… - Scientific reports, 2020 - nature.com
Electroporation is an electro-physical, non-viral approach to perform DNA, RNA, and protein
transfections of cells. Upon application of an electric field, the cell membrane is …

Tutorial: Electroporation of cells in complex materials and tissue

L Rems, D Miklavčič - Journal of Applied Physics, 2016 - pubs.aip.org
Electroporation is being successfully used in biology, medicine, food processing, and
biotechnology, and in some environmental applications. Recent applications also include in …

[HTML][HTML] Models of electroporation and the associated transmembrane molecular transport should be revisited

M Scuderi, J Dermol-Černe, CA da Silva… - …, 2022 - Elsevier
Electroporation has become a powerful tool for nonviral delivery of various biomolecules
such as nucleic acids, proteins, and chemotherapeutic drugs to virtually any living cell by …

[HTML][HTML] Multiple nanosecond electric pulses increase the number but not the size of long-lived nanopores in the cell membrane

AG Pakhomov, E Gianulis, PT Vernier… - … et Biophysica Acta (BBA …, 2015 - Elsevier
Exposure to intense, nanosecond-duration electric pulses (nsEP) opens small but long-lived
pores in the plasma membrane. We quantified the cell uptake of two membrane integrity …

Pulsed electric field inactivation of microorganisms: from fundamental biophysics to synergistic treatments

AL Garner - Applied microbiology and biotechnology, 2019 - Springer
The growth of antibiotic resistant microorganisms and the increasing demand for nonthermal
antimicrobial treatment in the food and beverage industry motivates research into alternative …

Microfluidic screening of electric fields for electroporation

PA Garcia, Z Ge, JL Moran, CR Buie - Scientific reports, 2016 - nature.com
Electroporation is commonly used to deliver molecules such as drugs, proteins, and/or DNA
into cells, but the mechanism remains poorly understood. In this work a rapid microfluidic …

Cell membrane electroporation modeling: A multiphysics approach

E Goldberg, C Suárez, M Alfonso, J Marchese… - …, 2018 - Elsevier
Electroporation-based techniques, ie techniques based on the perturbation of the cell
membrane through the application of electric pulses, are widely used at present in medicine …