The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

The widespread use of controlled molecular‐level motion in key natural processes suggests
that great rewards could come from bridging the gap between the present generation of …

Do biological motors move with regular steps? To address this question, we constructed
instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular …

A new in vitro assay using a feedback enhanced laser trap system allows direct
measurement of force and displacement that results from the interaction of a single myosin …

Cells employ a variety of linear motors, such as myosin1–3, kinesin4 and RNA polymerase5,
which move along and exert force on a filamentous structure. But only one rotary motor has …

Ca2+ regulation of contraction in vertebrate striated muscle is exerted primarily through
effects on the thin filament, which regulate strong cross-bridge binding to actin. Structural …

Myosin V is a dimeric molecular motor that moves processively on actin, with the center of
mass moving∼ 37 nanometers for each adenosine triphosphate hydrolyzed. We have …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …

The application of single-molecule fluorescence techniques to complex biological systems
places demands on the performance of single fluorophores. We present an enzymatic …

VISUALIZATION of single actin filaments by fluorescence microscopy1led to the
development of new in vitro assays for analysing actomyosin-based motility at the molecular …