Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is
still the dominant host for recombinant protein production but, as a bacterial cell, it also has …
still the dominant host for recombinant protein production but, as a bacterial cell, it also has …
Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications
CL Young, ZT Britton, AS Robinson - Biotechnology journal, 2012 - Wiley Online Library
Protein fusion tags are indispensible tools used to improve recombinant protein expression
yields, enable protein purification, and accelerate the characterization of protein structure …
yields, enable protein purification, and accelerate the characterization of protein structure …
Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily
The glutathione transferases (GSTs; also known as glutathione S-transferases) are major
phase II detoxification enzymes found mainly in the cytosol. In addition to their role in …
phase II detoxification enzymes found mainly in the cytosol. In addition to their role in …
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
K Terpe - Applied microbiology and biotechnology, 2003 - Springer
In response to the rapidly growing field of proteomics, the use of recombinant proteins has
increased greatly in recent years. Recombinant hybrids containing a polypeptide fusion …
increased greatly in recent years. Recombinant hybrids containing a polypeptide fusion …
Addition of p-Azido-l-phenylalanine to the Genetic Code of Escherichia coli
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in
vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response …
vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response …
Structure, catalytic mechanism, and evolution of the glutathione transferases
RN Armstrong - Chemical research in toxicology, 1997 - ACS Publications
The discovery in 1961 of a catalytic activity for the addition of glutathione (GSH) to 1, 2-
dichloro-4-nitrobenzene in cytosolic extracts of liver (1, 2) initiated three decades of …
dichloro-4-nitrobenzene in cytosolic extracts of liver (1, 2) initiated three decades of …
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
Benzophenones are among the most useful photocrosslinking agents in biology. We have
evolved an orthogonal aminoacyl-tRNA synthetase/tRNA pair that makes possible the in …
evolved an orthogonal aminoacyl-tRNA synthetase/tRNA pair that makes possible the in …
Glutathione transferases: a structural perspective
A Oakley - Drug metabolism reviews, 2011 - Taylor & Francis
The glutathione transferases (GSTs) are one of the most important families of detoxifying
enzymes in nature. The classic activity of the GSTs is conjugation of compounds with …
enzymes in nature. The classic activity of the GSTs is conjugation of compounds with …
Global rigid body modeling of macromolecular complexes against small-angle scattering data
MV Petoukhov, DI Svergun - Biophysical journal, 2005 - cell.com
New methods to automatically build models of macromolecular complexes from high-
resolution structures or homology models of their subunits or domains against x-ray or …
resolution structures or homology models of their subunits or domains against x-ray or …
Prion-like low complexity regions enable avid virus-host interactions during HIV-1 infection
Cellular proteins CPSF6, NUP153 and SEC24C play crucial roles in HIV-1 infection. While
weak interactions of short phenylalanine-glycine (FG) containing peptides with isolated …
weak interactions of short phenylalanine-glycine (FG) containing peptides with isolated …