Small RNA regulators (sRNAs) have been identified in a wide range of bacteria and found to
play critical regulatory roles in many processes. The major families of sRNAs include true …

The conventional view of transcription posits that mRNAs are generated from the coding
DNA strand and are delineated by gene boundaries; however, recent reports have mapped …

Antisense RNAs encoded on the DNA strand opposite another gene have the potential to
form extensive base-pairing interactions with the corresponding sense RNA. Unlike other …

Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …

Methods for simple and efficient regulation of metabolic pathway genes are essential for
maximizing product titers and conversion yields, and for minimizing the metabolic burden …

Abstract Information theory was used to build a promoter model that accounts for the− 10,
the− 35 and the uncertainty of the gap between them on a common scale. Helical face …

The vast majority of annotated transcripts in bacteria are mRNAs. Here we identify~ 1,000
antisense transcripts in the model bacterium Escherichia coli. We propose that these …

A surprise that has emerged from transcriptomics is the prevalence of genomic antisense
transcription, which occurs counter to gene orientation. While frequent, the roles of antisense …

Bacterial RNA polymerase is composed of a core of subunits (β β′, α1, α2, ω), which have
RNA synthesizing activity, and a specificity factor (σ), which identifies the start of transcription …

In synthetic biology, researchers assemble biological components in new ways to produce
systems with practical applications. One of these practical applications is control of the flow …