Scattering-based light microscopy: from metal nanoparticles to single proteins

L Priest, JS Peters, P Kukura - Chemical reviews, 2021 - ACS Publications
Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light
has undergone dramatic improvements over the past few decades. While fluorescence has …

Interferometric scattering microscopy: seeing single nanoparticles and molecules via Rayleigh scattering

RW Taylor, V Sandoghdar - Nano letters, 2019 - ACS Publications
Fluorescence microscopy has been the workhorse for investigating optical phenomena at
the nanometer scale but this approach confronts several fundamental limits. As a result …

Operando optical tracking of single-particle ion dynamics in batteries

AJ Merryweather, C Schnedermann, Q Jacquet… - Nature, 2021 - nature.com
The key to advancing lithium-ion battery technology—in particular, fast charging—is the
ability to follow and understand the dynamic processes occurring in functioning materials …

Self-supervised machine learning pushes the sensitivity limit in label-free detection of single proteins below 10 kDa

M Dahmardeh, H Mirzaalian Dastjerdi, H Mazal… - Nature …, 2023 - nature.com
Interferometric scattering (iSCAT) microscopy is a label-free optical method capable of
detecting single proteins, localizing their binding positions with nanometer precision, and …

Particle tracking of nanoparticles in soft matter

KA Rose, M Molaei, MJ Boyle, D Lee… - Journal of Applied …, 2020 - pubs.aip.org
Recent advances in optical microscopy instrumentation and processing techniques have led
to imaging that both breaks the diffraction barrier and enables sub-pixel resolution. This …

Point spread function in interferometric scattering microscopy (iSCAT). Part I: aberrations in defocusing and axial localization

R Gholami Mahmoodabadi, RW Taylor, M Kaller… - Optics …, 2020 - opg.optica.org
Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized
for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the …

Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications …

G Cui, DY Kim, D Zu, G Chai… - ACS Applied Nano …, 2024 - ACS Publications
The ability to spatially and temporally map nanoscale environments in situ over extended
time scales would be transformative for biology, biomedicine, and bioengineering. All …

Optical fingerprint of flat substrate surface and marker-free lateral displacement detection with angstrom-level precision

S Lin, Y He, D Feng, M Piliarik, XW Chen - Physical Review Letters, 2022 - APS
We report that flat substrates such as glass coverslips with surface roughness well below 0.5
nm feature notable speckle patterns when observed with high-sensitivity interference …

Adaptive dynamic range shift (ADRIFT) quantitative phase imaging

K Toda, M Tamamitsu, T Ideguchi - Light: Science & Applications, 2021 - nature.com
Quantitative phase imaging (QPI) with its high-contrast images of optical phase delay (OPD)
maps is often used for label-free single-cell analysis. Contrary to other imaging methods …

Enhancing nanoparticle detection in interferometric scattering (iSCAT) microscopy using a mask R-CNN

MJ Boyle, YE Goldman… - The Journal of Physical …, 2023 - ACS Publications
Interferometric scattering microscopy (iSCAT) is a label-free optical microscopy technique
that enables imaging of individual nano-objects such as nanoparticles, viruses, and …