Human topoisomerases and their roles in genome stability and organization
Y Pommier, A Nussenzweig, S Takeda… - … Reviews Molecular Cell …, 2022 - nature.com
Human topoisomerases comprise a family of six enzymes: two type IB (TOP1 and
mitochondrial TOP1 (TOP1MT), two type IIA (TOP2A and TOP2B) and two type IA (TOP3A …
mitochondrial TOP1 (TOP1MT), two type IIA (TOP2A and TOP2B) and two type IA (TOP3A …
Optical tweezers in single-molecule biophysics
CJ Bustamante, YR Chemla, S Liu… - Nature Reviews Methods …, 2021 - nature.com
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
Dynamic alternative DNA structures in biology and disease
G Wang, KM Vasquez - Nature Reviews Genetics, 2023 - nature.com
Repetitive elements in the human genome, once considered 'junk DNA', are now known to
adopt more than a dozen alternative (that is, non-B) DNA structures, such as self-annealed …
adopt more than a dozen alternative (that is, non-B) DNA structures, such as self-annealed …
DNA topoisomerases: Advances in understanding of cellular roles and multi‐protein complexes via structure‐function analysis
DNA topoisomerases, capable of manipulating DNA topology, are ubiquitous and
indispensable for cellular survival due to the numerous roles they play during DNA …
indispensable for cellular survival due to the numerous roles they play during DNA …
Topoisomerase-modulated genome-wide DNA supercoiling domains colocalize with nuclear compartments and regulate human gene expression
DNA supercoiling is a biophysical feature of the double helix with a pivotal role in biological
processes. However, understanding of DNA supercoiling in the chromatin remains limited …
processes. However, understanding of DNA supercoiling in the chromatin remains limited …
[HTML][HTML] MYC assembles and stimulates topoisomerases 1 and 2 in a “topoisome”
High-intensity transcription and replication supercoil DNA to levels that can impede or halt
these processes. As a potent transcription amplifier and replication accelerator, the proto …
these processes. As a potent transcription amplifier and replication accelerator, the proto …
Nucleosomes play a dual role in regulating transcription dynamics
Transcription has a mechanical component, as the translocation of the transcription
machinery or RNA polymerase (RNAP) on DNA or chromatin is dynamically coupled to the …
machinery or RNA polymerase (RNAP) on DNA or chromatin is dynamically coupled to the …
Supercoiling-mediated feedback rapidly couples and tunes transcription
CP Johnstone, KE Galloway - Cell reports, 2022 - cell.com
Transcription induces a wave of DNA supercoiling, altering the binding affinity of RNA
polymerases and reshaping the biochemical landscape of gene regulation. As supercoiling …
polymerases and reshaping the biochemical landscape of gene regulation. As supercoiling …
Etoposide promotes DNA loop trapping and barrier formation by topoisomerase II
Etoposide is a broadly employed chemotherapeutic and eukaryotic topoisomerase II poison
that stabilizes cleaved DNA intermediates to promote DNA breakage and cytotoxicity. How …
that stabilizes cleaved DNA intermediates to promote DNA breakage and cytotoxicity. How …
High-resolution, genome-wide mapping of positive supercoiling in chromosomes
Supercoiling impacts DNA replication, transcription, protein binding to DNA, and the three-
dimensional organization of chromosomes. However, there are currently no methods to …
dimensional organization of chromosomes. However, there are currently no methods to …