The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Tomato (Solanum lycopersicum L.), family Solanaceae, has become in the past fifty years
one of the most important and extensively grown horticultural crops in the Mediterranean …

Monkeypox, caused by the monkeypox virus (MPXV), is a zoonotic disease endemic mainly
in West and Central Africa. As of 27 September 2022, human monkeypox has occurred in …

Genome editing technology has rapidly evolved to knock-out genes, create targeted genetic
variation, install precise insertion/deletion and single nucleotide changes, and perform large …

Plant viruses cause significant losses in agricultural crops worldwide, affecting the yield and
quality of agricultural products. The emergence of novel viruses or variants through genetic …

Infectious pathogens cause severe human illnesses and great deaths per year worldwide.
Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing …

Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

The trans-cleavage activity of Cas12a has been widely used with various applications. Here,
we report that the trans-cleavage activity of Cas12a can be significantly affected by the …

The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to
address fundamental questions in every field of biological sciences has led to their …

Rapid, specific, and sensitive detection platforms are prerequisites for early pathogen
detection to efficiently contain and control the spread of contagious diseases. Robust and …