Many plants cannot vitrify themselves because they lack glassy state-inducing substances
and/or have high water content. Therefore, cryoprotectants are used to induce vitrification. A …

Spanish territories contain many of the hot spots of plant biodiversity among European
countries. Most of the Spanish territory is found in the Mediterranean basin and in the …

The effect of different cytokinins on multiple shoot regeneration from shoots of Centaurea
ultreiae was studied. The culture system consisted of solid basal half-strength Murashige …

Plant cryopreservation involves the storage of plant tissues (usually seed or shoot tips) in
liquid nitrogen (LN) at-196 C or in the vapour phase of LN at-135 C in such a way that the …

Cryogenic storage techniques have been developed and adopted for more than 100 (mainly
agricultural) plant species worldwide, and within Australia, at least 30 critically endangered …

Cryopreservation is one of the most promising techniques applied for the long-term
conservation of various plant genetic resources ex situ. Tremendous progress has been …

In this study conservation of Castilleja levisecta Greenm., a globally endangered species
was addressed through in vitro cryopreservation of shoot tips. In vitro cultures were …

In vitro culture is currently used to produce plant material for ex situ conservation of
endangered species. In this study, an efficient protocol for shoot regeneration from leaves …

This study provides alternative approaches toward ex situ conservation by means of in vitro
seed germination and the multiplication of Penthorum chinense Pursh using nodal explants …

Biodiversity of threatened species can be preserved in cryopreservation repositories,
allowing storage of biological information and future re-introduction to the wild. The success …