Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics
BA Graver, N Chakravarty, KV Solomon - Trends in Biotechnology, 2024 - cell.com
Prokaryotic Argonautes (pAgos) are an emerging class of programmable endonucleases
that are believed to be more flexible than existing CRISPR–Cas systems and have …
that are believed to be more flexible than existing CRISPR–Cas systems and have …
A predicted CRISPR-mediated symbiosis between uncultivated archaea
CRISPR–Cas systems defend prokaryotic cells from invasive DNA of viruses, plasmids and
other mobile genetic elements. Here, we show using metagenomics, metatranscriptomics …
other mobile genetic elements. Here, we show using metagenomics, metatranscriptomics …
CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications
In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …
Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing
G Bigelyte, B Duchovska, R Zedaveinyte… - Nucleic Acids …, 2024 - academic.oup.com
Cas9 and Cas12 nucleases of class 2 CRISPR-Cas systems provide immunity in
prokaryotes through RNA-guided cleavage of foreign DNA. Here we characterize a set of …
prokaryotes through RNA-guided cleavage of foreign DNA. Here we characterize a set of …
Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition
CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems.
We demonstrate that transposon protein TniQ plays a central role in promoting R-loop …
We demonstrate that transposon protein TniQ plays a central role in promoting R-loop …
Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System
AM Garza Elizondo, J Chappell - ACS Synthetic Biology, 2023 - ACS Publications
Synthetic perturbation of gene expression is central to our ability to reliably uncover
genotype–phenotype relationships in microbes. Here, we present a novel transcription …
genotype–phenotype relationships in microbes. Here, we present a novel transcription …
Type IV-A3 CRISPR-Cas systems drive inter-plasmid conflicts by acquiring spacers in trans
Plasmid-encoded type IV-A CRISPR-Cas systems lack an acquisition module, feature a
DinG helicase instead of a nuclease, and form ribonucleoprotein complexes of unknown …
DinG helicase instead of a nuclease, and form ribonucleoprotein complexes of unknown …
Interrogating two extensively self-targeting Type I CRISPR-Cas systems in Xanthomonas albilineans reveals distinct anti-CRISPR proteins that block DNA …
F Wimmer, F Englert, KG Wandera… - Nucleic Acids …, 2024 - academic.oup.com
CRISPR-Cas systems store fragments of invader DNA as spacers to recognize and clear
those same invaders in the future. Spacers can also be acquired from the host's genomic …
those same invaders in the future. Spacers can also be acquired from the host's genomic …
RNA-guided genome engineering: paradigm shift towards transposons
CRISPR-Cas systems revolutionized the genome engineering field but need to induce
double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size …
double-strand breaks (DSBs) and may be difficult to deliver due to their large protein size …
Distinct horizontal transfer mechanisms for type I and type V CRISPR-associated transposons
CASTs use both CRISPR-associated proteins and Tn7-family transposons for RNA-guided
vertical and horizontal transmission. CASTs encode minimal CRISPR arrays but can't …
vertical and horizontal transmission. CASTs encode minimal CRISPR arrays but can't …