Isothermal amplification of nucleic acids: The race for the next “gold standard”

BB Oliveira, B Veigas, PV Baptista - Frontiers in Sensors, 2021 - frontiersin.org
Nucleic acid amplification technologies (NAATs) have become fundamental tools in
molecular diagnostics, due to their ability to detect small amounts of target molecules. Since …

Point-of-care-testing of nucleic acids by microfluidics

L Zhang, B Ding, Q Chen, Q Feng, L Lin… - TrAC Trends in Analytical …, 2017 - Elsevier
Nucleic acid detection offers critical information in diagnosing pathogen infection and
monitoring disease treatment. Point-of-care testing (POCT), with ideal characteristics such …

Homogeneous circle-to-circle amplification for real-time optomagnetic detection of SARS-CoV-2 RdRp coding sequence

B Tian, F Gao, J Fock, M Dufva, MF Hansen - Biosensors and Bioelectronics, 2020 - Elsevier
Circle-to-circle amplification (C2CA) is a specific and precise cascade nucleic acid
amplification method consisting of more than one round of padlock probe ligation and rolling …

Integration of isothermal amplification methods in microfluidic devices: Recent advances

MC Giuffrida, G Spoto - Biosensors and Bioelectronics, 2017 - Elsevier
The integration of nucleic acids detection assays in microfluidic devices represents a highly
promising approach for the development of convenient, cheap and efficient diagnostic tools …

Multiplexed isothermal nucleic acid amplification

O Mayboroda, I Katakis, CK O'Sullivan - Analytical biochemistry, 2018 - Elsevier
Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …

Rolling circle amplification as an efficient analytical tool for rapid detection of contaminants in aqueous environments

K Zhang, H Zhang, H Cao, Y Jiang, K Mao, Z Yang - Biosensors, 2021 - mdpi.com
Environmental contaminants are a global concern, and an effective strategy for remediation
is to develop a rapid, on-site, and affordable monitoring method. However, this remains …

Rolling circle amplification as isothermal gene amplification in molecular diagnostics

NI Goo, DE Kim - Biochip journal, 2016 - Springer
Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an
efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic …

Advances in reagents storage and release in self‐contained point‐of‐care devices

J Deng, X Jiang - Advanced Materials Technologies, 2019 - Wiley Online Library
Microfluidics has emerged as a useful material for point‐of‐care testing. However,
conventional microfluidic devices rely on multiple steps to complete the detection process …

A novel rolling circle amplification-based detection of SARS-CoV-2 with multi-region padlock hybridization

R Kumari, JW Lim, MR Sullivan, R Malampy, C Baush… - Diagnostics, 2022 - mdpi.com
SARS-CoV-2 has remained a global health burden, primarily due to the continuous
evolution of different mutant strains. These mutations present challenges to the detection of …

Allele-specific ligation and recombinase polymerase amplification for the detection of single nucleotide polymorphisms

A Lazaro, ES Yamanaka, A Maquieira… - Sensors and Actuators B …, 2019 - Elsevier
A novel multiplex detection of single nucleotide polymorphisms (SNPs), with point-of-care
testing as its aim, is reported for supporting pharmacogenetic-based decisions. The strategy …